MM cells were incubated with 0, 5, 10, and 20 nM bortezomib for 12 h and MM cells were incubated with 10 nM bortezomib for 12, 24, and 36 h. Cells were then stained with Nectin-2-PE (BD Biosciences), PVR-PE, MICA-PE, and MICB-APC (R&D Systems, Minneapolis, MN, USA). Purified induced NK and γδ T cells were stained with CD56-FITC (BD Biosciences) or Vγ9-FITC, CD3-PerCp (BD Biosciences), NKG2D-APC (BD Biosciences), and DNAM-1-PE (BD Biosciences). Appropriate isotype-matched antibodies were used as controls. Data were analyzed using a BD FACSCalibur (BD Biosciences) with Cell Quest Pro software and final analysis was performed using FlowJo software (Tree Star Inc., Ashland, OR, USA).
Nectin 2 pe
Manufactured by BD
Sourced in United States
Nectin-2-PE is a fluorescently labeled antibody that binds to the Nectin-2 cell surface protein. Nectin-2 is an adhesion molecule involved in cell-cell interactions. The PE (Phycoerythrin) fluorophore is conjugated to the antibody, allowing for the detection and analysis of Nectin-2 expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Cd56 fitc, by BD (1 mentions)
Cd3 percp, by BD (1 mentions)
Vγ9 fitc, by BD (1 mentions)
Dnam 1 pe, by BD (1 mentions)
Nkg2d apc, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Vybrant cfda se cell tracer kit, by Thermo Fisher Scientific (1 mentions)
Cd107a apc, by BD (1 mentions)
7 aad viability staining solution, by BioLegend (1 mentions)
Ceacam1 pe, by R&D Systems (1 mentions)
Ulbp 2 5 6 pe, by R&D Systems (1 mentions)
Hla e pe, by BioLegend (1 mentions)
Cd56 bv421, by BD (1 mentions)
Nk cell isolation kit, by Miltenyi Biotec (1 mentions)
2 protocols using nectin 2 pe
1
Bortezomib Modulates NK and γδ T Cell Activation
2
NK Cell Phenotyping and Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ICAM2‐PE, nectin‐2‐PE, MICA/B‐PE HLA‐A, B, C‐PE CD3‐BV510, CD56‐BV421, and CD107a‐APC antibodies were purchased from BD Biosciences. CEACAM1‐PE, ULBP1‐APC, ULBP2/5/6‐PE, and ULBP3‐APC antibodies were purchased from R&D Systems. The 7‐AAD Viability Staining Solution, PVR‐PE, HLA‐E‐PE, and HLA‐G‐PE were purchased from BioLegend (San Diego, CA). The BAT3 antibody was purchased from Abcam (Cambridge, UK). The ICAM1‐PE antibody was purchased from Becton Dickinson Immunocytometry Systems (San Jose, CA). We purchased the NK Cell Isolation Kit from Miltenyi Biotec (California), the Vybrant CFDA SE Cell Tracer Kit from Life Technologies (Ontario, Canada), and the HCV nonstructural protein 3/4A protease inhibitor BILN2061 (BILN) from Acme Bioscience (Belmont, CA).19
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