Ethylenediamine n n n n tetraacetic acid

N-Octyl-β-d-glucoside (βOG),
ethylenediamine-N-N-N′-N′-tetraacetic
acid (EDTA), and 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic
acid (HEPES) were purchased from Dojindo Laboratories (Kumamoto, Japan).
Ethanol, isopropyl-β-d(−)-galactopyranoside
(IPTG), 2-amino-2-hydroxymethyl-1,3-propanediol (Tris), sodium phosphate,
lysozyme, sucrose, glucose, urea, glycine, NaCl, NaF, KCl, and CaCl₂ were purchased from Wako Pure Chemical Industries, Ltd. (Osaka,
Japan). Polyethylene glycol mono-p-isooctylphenyl
ether (Triton X-100) was purchased from Nacalai Tesque Inc., (Kyoto,
Japan). 1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DOPG) were purchased from Avanti
Polar Lipids Inc. (Alabaster, AL, USA). Alexa Fluor 546 carboxylic
acid, succinimidyl ester, Oregon Green BAPTA-5N, and hexapotassium
salt were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Calcein was purchased from Sigma-Aldrich (St. Louis, MO, USA). 5-Carboxyfluorescein
was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

AETS, TTAU, HTAU, and cysteamine hydrochloride were kindly gifted from Sogo Pharmaceutical Co., Ltd. (Tokyo, Japan). 5,5-Dimethyl-1-pyrroline-N-oxide (DMPO) was purchased from Labotec (Tokyo, Japan), xanthine oxidase (XOD) from bovine milk was from Sigma-Aldrich (St. Louis, MO), hypoxanthine (HX) and iron(II) sulfate heptahydrate were from FUJIFILM Wako Pure Chemical Co. (Osaka, Japan), heparin sodium salt solution was from Mochida Pharmaceutical Co., Ltd. (Tokyo, Japan), ethylenediamine-N,N,N’,N’-tetraacetic acid, disodium salt, dihydrate (EDTA) and diethylenetriamine-N,N,N’,N’’,N’’-pentaacetic acid (DTPA) were from Dojindo (Kumamoto, Japan). Pure water was freshly prepared with a Millipore Milli-Q Labo (Bedford, MA). All other reagents were of the highest purity commercially available.