Trans blot turbo ready to assemble mini 0.2 μm pvdf transfer kits

All of the chemicals were obtained from Fisher Scientific unless otherwise stated. The LB/carbenicillin plates, Tris-Glycine-SDS, and TBST buffers were from Teknova. The gels and Trans-Blot Turbo Ready-To-Assemble (RTA) Mini 0.2 μm PVDF Transfer Kits were from Bio-Rad. GST-VraS and Strep-VraR were probed in WB with GST Tag Antibody, HRP Conjugate (Invitrogen number MA4-004-HRP), and Strep-Tag II Antibody HRP Conjugate (EMD Millipore number 71591-M), respectively. For the kinase assay, the PK/LDH mixture was from Sigma, and the 96-well plates were from Corning (number 3695). The constructs of GST-VraS (UniProt accession number Q99SZ7) in a pGEX-4T-1 plasmid and Strep-VraR (UniProt accession number Q7A2Q1) in a p51b plasmid were generous gifts from Aurijit Sarkar.

All chemicals were obtained from Fisher Scientific unless otherwise stated. LB/carbenicillin plates, Tris-Glycine-SDS, and TBST buffers were from Teknova. 4–12% Tris-Glycine gel, Unstained Protein Standards, and Trans-Blot Turbo Ready-To-Assemble (RTA) Mini 0.2 μm PVDF Transfer Kits for Western blots (WB) were from BioRad. The kinases were probed with anti-His Tag Antibody HRP-labeled Mouse Monoclonal IgG (R&D Systems # MAB050H).