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Mouse monoclonal anti gst

Manufactured by Proteintech
Sourced in China

Mouse monoclonal anti-GST is a laboratory reagent used for the detection and purification of GST-tagged proteins. It is a mouse-derived monoclonal antibody that specifically binds to the glutathione S-transferase (GST) tag.

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3 protocols using mouse monoclonal anti gst

1

Evaluating Histone Modifications in Intrahepatic Cholangiocarcinoma

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Antibodies, including rabbit anti-H2A.Z (CST, Leiden, the Netherlands, 50722S), anti-H3K4me1 (CST, 5326S), rabbit polyclonal anti-Histone 3 (Proteintech, Chicago, USA, 17,168–1-AP), anti-H3K4me2 (CST, 9725S), anti-KDM1A (CST, 2139S), anti-SFRP1 (CST, 3534S), goat polyclonal anti-HP1α (Abcam, MA, USA, ab77256), mouse monoclonal anti-GST (Proteintech, 66,001–1-Ig), mouse monoclonal anti-GAPDH (Boster, Wuhan, China, BM1623) and IgG from healthy animals (Beyotime, Shanghai, China, A7007) were obtained from different companies.
Twenty-five pairs of human ICC and paracancerous tissues were collected from the Department of Biliary-Pancreatic Surgery at Tongji Hospital of Huazhong University of Science and Technology. With the written informed consent of individual patients, the experimental protocol was approved by the Tongji Hospital Ethics Committee.
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2

Comprehensive Profiling of ICC Epigenetics

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Antibodies included rabbit anti-CyclinD1 (Abcam, Cambridge, MA, USA, ab6152), anti-H3K9me3 (Abcam, ab8898), anti-CDK6 (Abcam, ab108357), polyclonal anti-SUV39H1 (Proteintech, Wuhan, China, 10,574–1-AP), mouse monoclonal anti-Dicer (Abcam, ab14601), rabbit polyclonal anti-Dnmt1 (Santa Cruz Biotech, Dallas, TX, USA, sc-20,701), polyclonal anti-Dnmt3a (Santa Cruz Biotech, sc-20,703), polyclonal anti-Dnmt3b (Santa Cruz Biotech, sc-20,704), SUV39H1 (Proteintech, 10,574–1-AP), goat polyclonal anti-HP1α (Abcam, ab77256), rabbit polyclonal anti-Histone3 (Proteintech, 17,168–1-AP), mouse monoclonal anti-β-actin (BOSTER, Wuhan, China, BM0626), mouse monoclonal anti-GST (Proteintech, 66,001–1-Ig) and IgG from healthy animals (Beyotime, Shanghai, China, A7007).
Fourteen pairs of human ICC and adjacent non-tumor tissues were obtained from the Department of Biliary-Pancreatic Surgery, Tongji Hospital of Huazhong University of Science and Technology (HUST, Hubei, China). Written informed consent was obtained from individual patients and the experimental protocol was approved by the Ethics Committee of the Tongji Hospital.
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3

Phytochemical Profiling of Specialty Crops

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Tea (C. sinensis L. cv. ‘Shuchazao’), oil tea (C. oleifera Abel.), grape (V. vinifera L.), and persimmon (D. kaki Thunb.) plants were cultivated in the experimental garden at Anhui Agricultural University (Anhui, China). Fresh samples were collected, rapidly frozen in liquid nitrogen, and stored at −80°C until use. Nicotiana tabacum cv. G28 (tobacco) and N. benthamiana were grown in a greenhouse with a light intensity of 150–200 μmol m−2 sec−1, a photoperiod of 16/8 h light/dark, and a temperature of 22 ± 2°C.
Authentic standard compounds, EC, EGC, ECG, EGCG, PGG, catechin, and gallocatechin were purchased from Sigma‐Aldrich (St. Louis, MO, USA). 1‐O‐β‐Glucogallin and caffeoyl glucoside were biosynthesized with CsUGT84A22 according to the methods in our previous report (Cui et al., 2016 (link)). EC‐Caf was provided by Prof. Guanhu Bao. Mouse monoclonal anti‐GFP was purchased from Abmart Inc. (Shanghai, China). Rabbit polyclonal anti‐plant actin was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Mouse monoclonal anti‐GST, mouse monoclonal anti‐MBP, and horseradish peroxidase‐conjugated secondary antibodies were purchased from Proteintech, Inc. (Wuhan, China).
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