Deoxyribonucleic acid sodium salt from

Reagents including, sodium hydroxide pellets, phosphate buffered saline (PBS) tablets, chloroform, magnesium chloride hexahydrate, Trizma (1 M), deoxyribonucleic acid sodium salt from calf thymus (ctDNA), 2'-deoxyadenosine monohydrate (dA), 2'- deoxycytidine hydrate (dC), thymidine (dT), 2'-deoxyguanosine hydrate (dG), DNase I (bovine pancreas), phosphodiesterase I (Crotalus adamanteus venom), alkaline phosphatase (Escherichia coli), were purchased from Sigma Aldrich (St. Louis, MO). Water, methanol, acetonitrile, ethanol, isopropanol, acetic and formic acids were purchased from Fisher Scientific (Pittsburg, PA) in the highest purity or LC-MS grade when available. The adduct (±)-anti- benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide-N²- [¹⁵N₅]-deoxyguanosine (¹⁵N-BaP-dG) was synthesized in our laboratory [27 ]. The activated carcinogen (±)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE) was purchased from MRI Global (Kansas City, MO). The Qiagen Blood and Cell Culture Midi^® Kit and the Quant-IT™ Qubit^® dsDNA BR assay kit were purchased from Invitrogen (Carlsbad, CA).