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Qpcr sybergreen master mix

Manufactured by Qiagen
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The QPCR SyberGreen master mix is a pre-formulated solution for real-time quantitative PCR (qPCR) analysis. It contains the necessary components for amplification and detection of DNA targets, including a DNA polymerase, buffer, and the SybrGreen fluorescent dye.

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Lab products found in correlation

Rt2 first strand kit, by Qiagen (2 mentions) Rneasy plus mini kit, by Qiagen (2 mentions) Mastercycler realplex 2 equipment, by Eppendorf (2 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

QPCR master mix (5 citations) SyberGreen (5 citations)

2 protocols using qpcr sybergreen master mix

1

Prostate Cancer Transcriptome Analysis

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Twenty five-week-old TRAMP/Akt1−/− and TRAMP/Akt1+/+ mice were treated with 10 mg/kg TCBN for 6 weeks, prostates were collected at 31 weeks and subjected to quantitative Real-Time PCR arrays. Briefly, tissues were lysed and RNA was isolated using RNeasy Mini Plus Kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol. Next cDNA was generated using RT2 First Strand Kit (SA Biosciences, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into Mouse PCa RT2 Profiler PCR Array plate (SA Biosciences, Frederick, MD). Reading was performed in Eppendorf Mastercycler realplex-2 equipment (Eppendorf, Hauppauge, NY).
Gao F., Alwhaibi A., Sabbineni H., Verma A., Eldahshan W, & Somanath P.R. (2017). Suppression of Akt1- β-catenin pathway in advanced prostate cancer promotes TGFβ1-mediated epithelial to mesenchymal transition and metastasis. Cancer letters, 402, 177-189.
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2

Gene Array Analysis of EMT in HMECs

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Control and ShAkt1 HMEC lysates were used for the gene array analysis using human epithelial to mesenchymal transition gene arrays. Briefly, cells were lysed and RNA was isolated using RNeasy Mini plus Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions and the quality was confirmed by using Nanodrop 2000 spectrophotometer (Thermo Scientific). Next, cDNA was generated by RT2 First Strand Kit (SABiosciences, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into human EMT RT2 Profiler PCR Array plates. Reading was completed in Eppendorf Mastercycler realplex-2 equipment (Hauppauge, NY).
Sabbineni H., Verma A., Artham S., Anderson D., Amaka O., Liu F., Narayanan S.P, & Somanath P.R. (2019). Pharmacological inhibition of β-catenin prevents EndMT in vitro and vascular remodeling in vivo resulting from endothelial Akt1 suppression. Biochemical pharmacology, 164, 205-215.
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