Shim park rp c18 column

The high-performance liquid chromatography (LC-20AT, Shimadzu) with an Agilent ODS column (4.6 × 250 mm) was used for HPLC analysis. Preparative HPLC (pHPLC) was carried out using Waters 2,767 coupled with a Shim-park RP-C18 column (250 × 20 mm i.d., Shimadzu). NMR spectra were measured in MeOD at 298 K on a Bruker Ascend^™ 500 M NMR (¹H NMR, 500 MHz; ¹³C NMR, 125 MHz) spectrometer with TMS as internal standard. HRMS (ESI) m/z measurements were obtained on a Thermo Scientific Q Exactive mass spectrometer. SEM photos were taken by an S3400n scanning electron microscopy (Hitachi). Software MEGA 5.05 was used to establish phylogenetic tree.

UV spectra were measured on a Shimadzu UV-1800 spectrophotometer. A Thermo Scientific Nicolet iN10 Microscope was used to obtain IR spectra. ECD spectra were measured on a JASCO J1500 CD spectrometer. NMR spectra were acquired in DMSO-d₆ at 303 K on a Bruker AVANCE III-500 (¹H NMR, 500 MHz; ¹³C NMR, 125 MHz) with TMS as the internal standard. HRESIMS data were acquired on a Thermo Q-Exactive orbitrap mass spectrometer. Preparative HPLC was performed on a Shimadzu HPLC system consisting of an LC-6AD pump and a Shimadzu SPD-20A detector, coupled with a Shim-park RP-C18 column (5 μm, 200 × 20 mm i.d., Shimadzu, Kyoto, Japan). A Lux Cellulose-2 column (5 μm, 250 × 4.6 mm i.d., Phenomenex, Torrance, CA, USA) was used for chiral separation. Silica gel (200–400 mesh, Qingdao Marine Chemical Co., Ltd.), ODS (40–63 μm, Fuji, Minato, Japan) and Sephadex LH-20 (Pharmacia, Stockholm, Sweden) were used for open column chromatography (CC).