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3 protocols using palmitic acid

1

Preparation and Characterization of Photodynamic Therapy Agents

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Phosphate-buffered saline (PBS), methylene blue (MB), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lauric acid (LA) and palmitic acid (PA) were purchased from SAMCHUN (Pyeongtaek, Korea). Glycerol monostearate (GMS) was purchased from Kanto Chemical Co, Japan. Inc. (Tokyo, Japan). Tween 80 (TW 80) was purchased from Dae Jung Co., Ltd. (Busan, Korea). Chlorophyll-a paste was purchased from Shandong Lanmo Biotech Co. Ltd. (Shanghai, China). Methylene chloride (CH2Cl2, MC) was purchased from Duksan Co. Ltd. (Ansan-si, Gyeonggi-do, Korea). DPBF was purchased from TCI Chemicals (Tokyo, Japan). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from WelGENE (Gyeongsan, Korea). Penicillin-streptomycin solution (100×) and fetal bovine serum (FBS) were purchased from BioWest (Nuaillé, France). The cancer cell lines (HeLa and A549) were obtained from the Korean Cell Line Bank (Seoul, Korea). The Quanti-MAX WST-8 assay kit was purchased from Biomax (Seoul, Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) was purchased from Honeywell (Seelze, Germany). All other chemicals used were of HPLC grade.
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2

Formulation and Evaluation of Lipid Nanocarriers

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Cur and phosphate-buffered saline (PBS) were
purchased from Sigma-Aldrich (St. Louis, MO, USA). Lauric acid (LA),
palmitic acid (PA), and stearic acid (SA) were supplied by SAMCHUN
(Pyeongtaek, Korea). Poloxamer 188 (PX 188) and poloxamer 407 (PX
407) were purchased from BASF (Ludwigshafen, Germany). Tween 20 (TW
20) and Tween 80 (TW 80) were purchased from Dae Jung Co., Ltd. (Busan,
Korea). Dulbecco’s modified Eagle medium (DMEM) was supplied
by WelGENE (Gyeongsan, South Korea). Penicillin–streptomycin
solution (100×) and fetal bovine serum (FBS) were purchased from
BioWest (Nuaillé, France). The cancer cell lines (HeLa, A549,
and CT-26) were purchased from the Korea Cell Line Bank (Seoul, Korea).
The Quanti-MAX WST-8 assay kit was purchased from Biomax (Seoul, Korea).
High-performance liquid chromatography (HPLC) grade methanol (MeOH)
was purchased from Honeywell (Seelze, Germany). All compounds are
>95% pure by HPLC analysis.
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3

Formulation and Characterization of Photosensitizer-Loaded Nanoparticles

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Phosphate-buffered saline (PBS), methylene blue (MB), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Palmitic acid (PA) and stearic acid (SA) were supplied by SAMCHUN (Pyeongtaek, Korea). Glycerol monostearate (GMS) was obtained from Kanto Chemical Co, Japan. Inc. (Tokyo, Japan), and Poloxamers (PX) 188 and PX 407 were procured from BASF (Ludwigshafen, Germany). Tween 80 (TW 80) was purchased from Dae Jung Co., Ltd. (Busan, Korea) and chlorophyll-a paste from Shandong Lanmo Biotech Co. Ltd. (Shanghai, China). Methylene chloride (CH2Cl2, MC) and DPBF were supplied by Duksan Co. Ltd. (Gyeonggi-do, Korea) and TCI Chemicals (Tokyo, Japan), respectively. Dulbecco’s modified Eagle’s medium (DMEM) was provided by WelGENE (Gyeongsan, South Korea), and penicillin–streptomycin solution (100 ×) and fetal bovine serum (FBS) were purchased from BioWest (Nuaillé, France). The cancer cell lines (HeLa and A549) were obtained from the Korea Cell Line Bank (Seoul, Korea), and the Quanti-MAX WST-8 assay kit from Biomax (Seoul, Republic of Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) was purchased from Honeywell (Seelze, Germany). All other chemicals used were of HPLC grade.
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