All cell lines used in this work were obtained from American Type Culture Collection (Manassas, VA, USA). Cells were cultured as we previously described [42 (link)].
TGF-β was purchased from R&D systems (Minneapolis, MN, USA). SKF96365 was bought from Abcam (Abcam, UK). The calcium dye Fura 2-AM and EGTA were obtained from Invitrogen (Carlsbad, CA, USA). The antibodies against ERK1/2, p-ERK1/2 were bought from Bioworld Technology (Louis Park, MN, USA) and anti-STIM1 antibody was from Abcam (Abcam, UK). The anti-GAPDH antibody was bought from KANGCHEN BIO-TECH (Shanghai, China). HRP-conjugated goat anti-rabbit antibody was purchased from Thermo Scientific (Waltham, MA, USA).
TGF-β was purchased from R&D systems (Minneapolis, MN, USA). SKF96365 was bought from Abcam (Abcam, UK). The calcium dye Fura 2-AM and EGTA were obtained from Invitrogen (Carlsbad, CA, USA). The antibodies against ERK1/2, p-ERK1/2 were bought from Bioworld Technology (Louis Park, MN, USA) and anti-STIM1 antibody was from Abcam (Abcam, UK). The anti-GAPDH antibody was bought from KANGCHEN BIO-TECH (Shanghai, China). HRP-conjugated goat anti-rabbit antibody was purchased from Thermo Scientific (Waltham, MA, USA).