Fusion360 and SketchUp computer aided design (CAD) software was used for designing the MAs and designs were exported to an object file (.stl) using 3D Sprint (3D Systems, Inc., Rock Hill, SC, USA). The print resolution is highest in the z direction which is 0.02 mm. MA holders were 3D printed using a commercially available ProJet MJP 2500 printer (3D Systems, Inc.) using a VisiJet® M2R-CLR build material and a VisiJet® M2 SUP support material, both from 3D Systems. After printing, the MA holders were placed at −20 °C for 5 min to release the printed holders from the base plate. MA holders were then placed in a steam bath for 15 min to remove the wax support material and subsequently placed in a hot oil bath for another 15 min to remove all traces of the wax support. MA holders were then cleaned using hot tap water and soap and left at room temperature to dry.
Visijet m2 sup
Manufactured by 3D Systems
Sourced in United States
The VisiJet® M2 SUP is a 3D printing material designed for use with 3D Systems' additive manufacturing equipment. It is a support material that is intended to be used in conjunction with other build materials to facilitate the production of complex 3D printed parts.
Automatically generated - may contain errors
2 protocols using visijet m2 sup
1
3D Printing of Microarray Holders
2
3D-Printed Microfluidic Device Fabrication
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All 3D models were designed using the 3D-CAD-program SOLIDWORKS (Dassault Systèmes SOLIDWORKS Corporation). The multijet printer ProJet® MJP 2500 Plus (3D Systems, Rock Hill, SC, USA) was then used to produce the microfluidic devices. The polyacrylate photocurable resin VisiJet® M2R-CL (3D Systems, Rock Hill, SC, USA) was used to print the actually device, and hydroxylated wax (VisiJet® M2 SUP, 3D Systems, Rock Hill, SC, USA) acted as support material. 19 The printer was operated in HD mode, with a nominal resolution of 800 × 900 × 790 dpi and a layer resolution of 32 μm.
After printing, the device underwent several postprocessing steps according to Siller et al.: first, the printed parts are cooled at -18 °C for 5 minutes, followed by incubation in a hot water vapor bath, a hot ultrasonic oil bath and lastly in a hot ultrasonic water bath with detergent. 20
After printing, the device underwent several postprocessing steps according to Siller et al.: first, the printed parts are cooled at -18 °C for 5 minutes, followed by incubation in a hot water vapor bath, a hot ultrasonic oil bath and lastly in a hot ultrasonic water bath with detergent. 20
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