The pLV-U6-EGFP-Puro lentiviral plasmid was purchased from Inovogen Tech Co.
(Beijing, China). Three different cDNA fragments encoding BRF1 shRNAs were cloned downstream of the U6 promoter within the plasmid. The pGL3-basic plasmid containing a reporter gene was purchased from Promega Co. SaOS2, HeLa, 293T and M2 cell lines were obtained from ATCC. and cultured in their respective media with the supplement of 10% fetal bovine serum (Biowest Co.) and 1× penicillin/streptomycin (Hyclone Co.). DNA and RNA extraction kits were purchased from Axygene Co. Biological reagents, including restriction enzymes, reverse reuse, remix, or adapt this material for any purpose without crediting the original authors.
transcription enzyme, PCR, and transfection reagents, were obtained from Thermo Fisher Scientific Co. All other chemicals were purchased from Sigma-Aldrich (Merck).
(Beijing, China). Three different cDNA fragments encoding BRF1 shRNAs were cloned downstream of the U6 promoter within the plasmid. The pGL3-basic plasmid containing a reporter gene was purchased from Promega Co. SaOS2, HeLa, 293T and M2 cell lines were obtained from ATCC. and cultured in their respective media with the supplement of 10% fetal bovine serum (Biowest Co.) and 1× penicillin/streptomycin (Hyclone Co.). DNA and RNA extraction kits were purchased from Axygene Co. Biological reagents, including restriction enzymes, reverse reuse, remix, or adapt this material for any purpose without crediting the original authors.
transcription enzyme, PCR, and transfection reagents, were obtained from Thermo Fisher Scientific Co. All other chemicals were purchased from Sigma-Aldrich (Merck).