Log In Sign up
The largest database of trusted experimental protocols

Anti rabbit af568

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United Kingdom

Anti-rabbit AF568 is a fluorescently labeled secondary antibody designed to detect and visualize rabbit primary antibodies in various immunoassay applications. The dye, Alexa Fluor 568, provides bright and photostable fluorescence when excited at the appropriate wavelength.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit af488, by Thermo Fisher Scientific (2 mentions) Lsm780 confocal microscope system, by Zeiss (1 mentions) Rad51, by Abcam (1 mentions) Anti rat af488, by Thermo Fisher Scientific (1 mentions) Af 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Streptavidin af647, by Thermo Fisher Scientific (1 mentions) Af568 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Anti pkb t308 clone c25e6, by Cell Signaling Technology (1 mentions) Mirax midi slide scanner, by Zeiss (1 mentions) Anti ng2, by Merck Group (1 mentions) Pannoramic viewer 1, by 3DHISTECH (1 mentions) Anti cd31, by BioLegend (1 mentions) Lycopersicon esculentum tomato lectin, by Vector Laboratories (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Pimonidazole, by Hypoxyprobe (1 mentions) Anti gfap, by Synaptic Systems (1 mentions) Goat anti mouse 170 6516, by Bio-Rad (1 mentions) Anti β actin, by Novus Biologicals (1 mentions) Anti gephyrin, by Synaptic Systems (1 mentions)

4 protocols using anti rabbit af568

1

Quantifying RAD51 Foci in Irradiated Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
CL-V4B cells transduced with WT or RAD51C missense variant lentivirus were irradiated with γ-IR (5 Gy) and after 6 hours were fixed with 2% paraformaldehyde. Cells were incubated with primary antibodies [RAD51C (IgG1 mouse monoclonal, Santa Cruz Biotechnology), geminin (IgG2b mouse monoclonal, Santa Cruz Biotechnology) and RAD51 (rabbit polyclonal, Abcam)]. Cells were subsequently incubated with secondary antibodies [anti-mouse IgG1 AF488, anti-mouse IgG2b AF647 and anti-rabbit AF568 (Thermo Fisher Scientific)] for 1 hour. Z stack images were acquired using an inverted Zeiss LSM 780 confocal microscope system. Geminin-high cells (n = 1,000) positive and negative for RAD51 foci were counted and the mean percentage of geminin-high cells positive for RAD51 foci was plotted (Supplementary Fig. S1). All variants were analyzed in three independent experiments.
Hu C., Nagaraj A.B., Shimelis H., Montalban G., Lee K.Y., Huang H., Lumby C.A., Na J., Susswein L.R., Roberts M.E., Marshall M.L., Hiraki S., LaDuca H., Chao E., Yussuf A., Pesaran T., Neuhausen S.L., Haiman C.A., Kraft P., Lindstrom S., Palmer J.R., Teras L.R., Vachon C.M., Yao S., Ong I., Nathanson K.L., Weitzel J.N., Boddicker N., Gnanaolivu R., Polley E.C., Mer G., Cui G., Karam R., Richardson M.E., Domchek S.M., Yadav S., Hruska K.S., Dolinsky J., Weroha S.J., Hart S.N., Simard J., Masson J.Y., Pang Y.P, & Couch F.J. (2023). Functional and Clinical Characterization of Variants of Uncertain Significance Identifies a Hotspot for Inactivating Missense Variants in RAD51C. Cancer Research, 83(15), 2557-2571.
+ Open protocol
+ Expand
2

Comprehensive Immunophenotyping Approach

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-HSP90 (clone 3H3C27), anti-SHIP1 (clone PICI-A5), FITC-conjugated rat anti-mouse GR1 (clone RB6-8C5), PE-conjugated rat anti-mouse/human CD11b (clone M1/70), PE/Cy7-conjugated rat anti mouse/human CD45 (clone 30-F11), PerCP/Cy5.5-conjugated rat anti F4/80 (clone BM8), APC-conjugated rat anti-B220 (clone RA3-6B2), PE/Cy7-conjugated rat anti CD3 (clone 17A2) and pacific blue-conjugated rat anti-LY6G (clone 1A8) were from BioLegend (London, UK); anti-PTEN (clone D4.3), anti-PKB (clone 11E7), anti-PKB T308 (clone C25E6) and anti-PKB S473 (clone D9E) were from Cell Signaling Technology (London, UK). Rabbit IgG (I8140) was obtained from Sigma. Anti-SHIP2 (AF5389) and PE-conjugated rat anti-CD64 (clone FAB20741P) were from R&D Systems (Abingdon, UK) and biotinylated anti-PI(3,4)P2 (z-B034) was from Echelon Biosciences (Salt Lake City, UT, USA); streptavidin-AF647, AF488-conjugated phalloidin, AF568-conjugated phalloidin, and secondary antibodies anti-rat AF488, anti-rabbit AF568 and anti-rabbit AF488 were obtained from Thermo Fisher Scientific (Loughborough, UK).
Michael M., McCormick B., Anderson K.E., Karmakar U., Vermeren M., Schurmans S., Amour A, & Vermeren S. (2021). The 5-Phosphatase SHIP2 Promotes Neutrophil Chemotaxis and Recruitment. Frontiers in Immunology, 12, 671756.
+ Open protocol
+ Expand
3

Hypoxia and Vascular Perfusion Imaging in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were i.v. injected with 1.5 mg pimonidazole (Hypoxyprobe) one hour before termination. For determination of tissue perfusion, mice were i.v. injected with 100 µg FITC-labeled Lycopersicon esculentum (tomato) lectin (Vector Labs) 5 min prior to whole body perfusion with PBS followed by tumor dissection and embedding in OCT (TissueTek). Tissue sections (5 µm) were fixed for 10 min in ice-cold acetone, rehydrated in PBS and blocked with 1%BSA in PBST (PBS/0.1% Tween 20) for one hour at room temperature (RT). Sections were then incubated with anti-pimonidazole (Omni Kit Hypoxyprobe) and anti-CD31 (Biolegend) antibodies overnight at 4 °C in blocking buffer. After washing with PBST (3×), antibodies: anti-rabbit-AF568, anti-rat-AF647 (Life Technologies) were incubated for 1 h at RT, counterstained with DAPI (Sigma) and mounted in Prolong Gold (Life Technologies). Pericytes were stained with anti-NG2 (Millipore). pimonidazole, CD31 or NG2 staining was determined using MIRAX MIDI Slide Scanner (Zeiss). Images were acquired with a CLSM SP5 Resonant APD Confocal Microscope (Leica) and analyzed with the Imaris software (Bitplane). Vessel area was calculated using the software Pannoramic Viewer 1.15.2 (3D Histech).
Glaus Garzon J.F., Pastrello C., Jurisica I., Hottiger M.O., Wenger R.H, & Borsig L. (2020). Tumor cell endogenous HIF-1α activity induces aberrant angiogenesis and interacts with TRAF6 pathway required for colorectal cancer development. Neoplasia (New York, N.Y.), 22(12), 745-758.
+ Open protocol
+ Expand
4

Characterization of zDHHC15 Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used were: anti-zDHHC15 (Abcam, Cambridge, MA, #ab121203, 1:250, western blot), anti-zDHHC15 (Thermo Scientific, Waltham, MA, #PA39327, immunofluorescence, 1:100), anti-Flag (Sigma Aldrich, St Louis, MO, #F3165, 1:1000), anti-Myc (Origene, Rockville, MD, #TA150121, 1:1000), anti-β-actin (Novus Biologicals, Centennial, CO, #NB600-503, 1:1000), anti-PSD-95 (Abcam, Cambridge, MA, #ab2723, 1:500), anti-gephyrin (Synaptic Systems, Göttingen, Germany #147011, 1:500), anti-GAD65 (Synaptic Systems, Göttingen, Germany #198104, 1:500), anti-GFAP (Synaptic Systems, Göttingen, Germany #173004, 1:400), anti-MAP2 (Millipore, Burlington, MA, MB3418, 1:2000), anti-giantin (Synaptic Systems, Göttingen, Germany, #263004, 1:500), anti-GM130 (BD Biosciences, Franklin Lakes, NJ, #610822, 1:200). Secondary antibodies from Life Technologies (Carlsbad, CA,) used: (1:500), anti-mouse AF568 IgG2a (#A21134), anti-mouse AF647 IgG1 (#21240), anti-rabbit AF488 (#A11008), anti-rabbit AF568 (#A11011), anti-guinea pig AF633 (#A21105). HRP conjugated secondary antibodies were obtained from Bio-Rad (Hercules, CA): goat anti-mouse #170-6516 and goat anti-rabbit (#170-6515, 1:300). DAPI was used for nuclear staining (Life Technologies, Carlsbad, CA, #D1306, 1:1000).
Shah B.S., Shimell J.J, & Bamji S.X. (2019). Regulation of dendrite morphology and excitatory synapse formation by zDHHC15. Journal of Cell Science, 132(13), jcs230052.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!