Dl methionine

Dithiothreitol, Glutathione, N-acetyl l-cysteine, Disodium EDTA, Deferoxamine, DCFHDA, and 9,10 Phenanthrenequinone were purchased from Sigma Aldrich. The BODIPY C11 581/591 (Cat no #D3861) was purchased from Invitrogen-Thermo scientific. 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine, antibody MB47 and monoclonal antibody E06 were purchased from Avanti Polar Lipids. Ascorbic acid was purchased from SRL chemical Pvt. Ltd. The IL-6 ELISA kit was purchased from Invitrogen-Thermo scientific. dl-Methionine and D-mannitol was purchased from S.D. Fine- Chem Limited, Mumbai, India. Clinical surfactant, Neosurf (Bovine Lipid Extract Surfactant Suspension) manufactured by Cipla Pharmaceutical company was purchased from local medical store. The secondary antibody goat anti-mouse IgM-HRP (Cat no #ab97230) was purchased from Abcam.

The chemicals and reagents used in this study were of analytical grade. Cellulose powder, concentrated HCl, dl-methionine, ethanol, FeCl₃, sucrose, heptane, and n-butanol were from S.D. Fine Chemicals Ltd. (Mumbai, India). The total cholesterol (TC), serum glutamate-pyruvate transaminase (SGPT), serum glutamate-oxalate transaminase (SGOT), triglyceride (TG), and creatinine measurement kits were obtained from Span Diagnostics (Surat, India); the glucose and total protein measurement kits were obtained from Beacon Diagnostics (Navsari, India). Glucose was obtained from Avecon Health Care Pvt. Ltd. (Parwanoo, India) and the fasting insulin chemiluminescent immunoassay kit was from Diagnostic Automation Inc (Woodland Hills, CA, USA). The leptin enzyme-linked immunosorbent assay (ELISA), and MCP-1 and plasma resistin quantitation kits were from Ray Bio Rat (Norcross, GA, USA). A rat adiponectin Assay Max ELISA kit was obtained from Assay pro (St. Charles, MO, USA).