For transmission electron microscopy work, mouse skin tissue samples were cut to a minimal size (1 mm x 1mm) and fixed with 2 % glutaraldehyde/2 % paraformaldehyde in 0.1M cacodylate buffer, pH 7.4. Further processing was done according using standard protocols, including a number of optimized steps for skin tissue, as described previously40 (link). Resulting 60 nm ultrathin sections were analysed with a Tecnai 12-biotwin transmission electron microscope (ThermoFisher) and representative images were taken with a 2K CCD Veleta camera (EMSIS, Münster, Germany).
2k ccd veleta camera
Manufactured by EMSIS
Sourced in Germany
The 2K CCD Veleta camera is a high-resolution digital imaging device capable of capturing detailed images. It features a 2048 x 2048 pixel CCD sensor that provides a resolution of 4 megapixels. The camera is designed for laboratory and scientific applications that require precise and accurate image capture.
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Lab products found in correlation
2 protocols using 2k ccd veleta camera
1
Optimized TEM Protocol for Mouse Skin
2
Ultrastructural Tissue Fixation and Imaging
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Embryos were fixed in 2% PFA, 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.4. Samples were embedded in 2% LMP-agarose and postfixed in 1% osmium tetroxide, 1.5% potassium ferrocyanide in 0.1M cacodylate buffer. After that, the samples were dehydrated stepwise in ethanol, including en bloc 0.5% uranyl acetate staining during 70% ethanol. The last dehydration step was performed in propylene oxide followed by epon embedding. The samples were sectioned stepwise on an ultramicrotome (UC6, Leica) until the region of interest and 60-nm ultrathin sections were collected on a formvar-coated one-slot grid and counterstained with lead. The samples were imaged on a FEI-Tecnai 12-electron microscope at 80 kV (Thermo Fisher Scientific), and characteristic images were taken with a 2 K CCD-Veleta camera (EMSIS).
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