1 palmitoyl 2 arachidonoyl sn glycero 3 phosphocholine

Manufactured by Avanti Polar Lipids

Sourced in United States

1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine is a phospholipid compound. It is a naturally occurring lipid molecule found in cell membranes.

Automatically generated - may contain errors

Lab products found in correlation

Deferoxamine, by Merck Group (1 mentions) Dl methionine, by SD Fine Chemicals (1 mentions) 9 10 phenanthrenequinone, by Merck Group (1 mentions) Goat anti mouse igm hrp, by Abcam (1 mentions) Dcfh da, by Merck Group (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) Phenazine methosulfate, by Thermo Fisher Scientific (1 mentions) 2 3 bis 2 methoxy 4 nitro 5 sulfophenyl 2h tetrazolium 5 carboxanilide xtt, by Thermo Fisher Scientific (1 mentions) Hplc grade solvents, by Avantor (1 mentions) 1 heptadecanoyl 2hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 docosahexaenoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 linoleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 azelaoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 linoleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 5 oxo valeroyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 arachidonoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)

5 protocols using 1 palmitoyl 2 arachidonoyl sn glycero 3 phosphocholine

Lipid Composition Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following phospholipids were all purchased from Avanti Polar Lipids (Alabaster, AL): 1-palmitoyl-2-oleyol-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-eicosapentaenoyl-sn-glycero-3-phosphocholine (PL-EPA), 1-palmitoyl-2-docosahexaenyol-sn-glycero-3-phosphocholine (PL-DHA), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PL-AA). Each phospholipid examined in this study had the same head group moiety (phosphatidyl choline) and same acyl chain at the sn-1 position (palmitic acid). Powder cholesterol (from ovine wool) was also purchased from Avanti Polar Lipids. All lipids were dissolved in HPLC grade chloroform and stored at −20°C under nitrogen gas.

Sherratt S.C., Juliano R.A., Copland C., Bhatt D.L., Libby P, & Mason R.P. (2021). EPA and DHA containing phospholipids have contrasting effects on membrane structure. Journal of Lipid Research, 62, 100106.

+ Open protocol

+ Expand

Lipid Oxidation and Inflammation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dithiothreitol, Glutathione, N-acetyl l-cysteine, Disodium EDTA, Deferoxamine, DCFHDA, and 9,10 Phenanthrenequinone were purchased from Sigma Aldrich. The BODIPY C11 581/591 (Cat no #D3861) was purchased from Invitrogen-Thermo scientific. 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine, antibody MB47 and monoclonal antibody E06 were purchased from Avanti Polar Lipids. Ascorbic acid was purchased from SRL chemical Pvt. Ltd. The IL-6 ELISA kit was purchased from Invitrogen-Thermo scientific. dl-Methionine and D-mannitol was purchased from S.D. Fine- Chem Limited, Mumbai, India. Clinical surfactant, Neosurf (Bovine Lipid Extract Surfactant Suspension) manufactured by Cipla Pharmaceutical company was purchased from local medical store. The secondary antibody goat anti-mouse IgM-HRP (Cat no #ab97230) was purchased from Abcam.

Dey S.K., Sugur K., Venkatareddy V.G., Rajeev P., Gupta T, & Thimmulappa R.K. (2021). Lipid peroxidation index of particulate matter: Novel metric for quantifying intrinsic oxidative potential and predicting toxic responses. Redox Biology, 48, 102189.

+ Open protocol

+ Expand

Oxidative Phospholipid Transformation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

1-Palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids; Alabaster, AL, USA) was oxidized by exposure to air for 24 h until approx. 20% of the phospholipid remained intact. The oxidation progress was monitored by thin-layer chromatography and by mass spectrometry [8 (link)].

Oskolkova O.V., Godschachner V, & Bochkov V.N. (2017). Off-Target Anti-Inflammatory Activity of the P2X7 Receptor Antagonist AZ11645373. Inflammation, 40(2), 530-536.

+ Open protocol

+ Expand

Anti-inflammatory natural product evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plant-derived natural products and synthetic molecules with known or expected anti-inflammatory activity were purchased from different vendors. HA15 ((N-[4-[3-[[5-(dimethyl amino) naphthalen-1-yl]sulfonyl amino]phenyl]-1,3-thiazol-2-yl] acetamide, Calbiochem) was purchased from Sigma-Aldrich (St. Luis, MS). Geranylgeranyl acetone (GGA) and sodium salt of phenyl butyrate (PBA) were from Selleck Chemicals GmbH (Berlin, Germany). 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and phenazine methosulfate (PMS) were from Thermo Fisher Scientific (Waltham, MA, USA) or (TCI, Tokyo, Japan), respectively. OxPAPC was prepared from 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, Birmingham, AL, USA) and analysed as described (Watson et al. 1997 (link)). OxPAPC was stored as a chloroform solution at − 80 °C, and evaporated under the stream of argon before solubilisation in the cell culture medium.

Hellauer K., Oskolkova O.V., Gesslbauer B, & Bochkov V. (2023). Pharmacological heat-shock protein inducers and chemical chaperones inhibit upregulation of interleukin-8 by oxidized phospholipids. Inflammopharmacology, 31(3), 1319-1327.

+ Open protocol

+ Expand

Phospholipid Standards for Lipidomics

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC grade solvents were obtained from VWR International (Mississauga, Ontario, Canada). The standard phospholipids; 1,2-dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (17:0 LPC), 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC), 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (PAPC), 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (PDHPC), 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), 1-palmitoyl-2-azelaoyl-sn-glycero-3-phosphocholine (PAzPC) and 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine (PONPC) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC), 1-palmitoyl-2-(5’-keto-6’-octenedioyl)-sn-glycero-3-phosphocholine (KOdiA-PC) and 1-palmitoyl-2(4’-keto-dodec-3’-ene-dioyl)-sn-glycero-3-phosphocholine (KDdiA-PC) were purchased from Cayman Chemicals (Ann Arbor, Michigan, USA). All other chemicals were purchased from Sigma Aldrich (St. Louis, Missouri, USA).

Solati Z., Edel A.L., Shang Y., O K, & Ravandi A. (2018). Oxidized phosphatidylcholines are produced in renal ischemia reperfusion injury. PLoS ONE, 13(4), e0195172.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!