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Trehalose dihydrate

Manufactured by Sisco Research Laboratories
Sourced in India, Netherlands

Trehalose dihydrate is a naturally occurring disaccharide compound. It functions as a protective agent, stabilizing proteins, enzymes, and other biological molecules against various stresses, such as heat, cold, and desiccation.

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2 protocols using trehalose dihydrate

1

Chitosan-based Antimicrobial Nanoparticles

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Luria Bertini (LB), TMG buffer (Tris hydrochloride, magnesium sulfate, gelatin buffer), Muller Hinton Agar (MHA), Agar powder (Bacteriological grade), all antibiotic discs, and media were supplied by Himedia, Maharashtra, India. Trehalose dihydrate, chitosan oligosaccharide (extra pure, 90% degree of deacetylation), and sodium tripolyphosphate (STPP) were purchased from Sisco Research Laboratories, Mumbai, India. Glycerol (molecular biology grade, extra pure), DiD (DiIC18(5); 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt) were purchased from, Thermo Fisher, Waltham, MA, USA. All other chemicals and reagents used in this study were molecular biology grade, bacteriological, and lab grade.
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2

Mucoadhesive Nanoparticle-Based Delivery

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The following chemicals were used: Egyptian propolis (Mansoura, Province of Dakhahlia, Delta, Egypt), PDLGA®5010 (DL-lactide/glycolide 50/50, 153 KDa, Corbion, Amsterdam, the Netherlands), trehalose dihydrate (Sisco Research Laboratories Pvt Ltd, Mumbai, India), polyvinyl alcohol (PVA, 14 kDa, BDH, USA), hydroxypropyl methylcellulose (HPMC), Carbopol 940 (Colorcon Ltd, Orpington, UK), methylene blue (MB) (Sigma-Aldrich, St Louis, Missouri, USA), AH Plus® as a model of approved root canal sealers (Dentsply De Trey GmbH, Konstanz, Germany), nitric acid 69% extra pure, and thymol (Alpha Chemika, Mumbai, India), and anti-PCNA primary antibody (PC10) (Santa Cruz Biotechnology, Inc., Santa Cruz, California, USA). All other solvents and chemicals were of analytical grades.
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