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Penicillin streptomycin solution

Manufactured by Gemini Bio
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Penicillin-streptomycin solution is a commonly used antibiotic mixture. It contains the antibiotics penicillin and streptomycin, which are used to prevent bacterial contamination in cell culture and related applications.

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Lab products found in correlation

Prestained protein marker, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Ecl solution, by Thermo Fisher Scientific (1 mentions) Lactate dehydrogenase ldh assay kit, by Nanjing Jiancheng (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Genipin, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Nanjing Jiancheng (1 mentions) Caspase 3 assay kit, by Beyotime (1 mentions) Glutathione peroxidase gsh px, by Nanjing Jiancheng (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Countess 2 automated cell counter, by Thermo Fisher Scientific (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Neurobasal media, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions) 100 μm cell strainer, by Avantor (1 mentions) Papain, by Worthington (1 mentions)

Close spelling variants of this product

Penicillin/streptomycin (97 citations) Streptomycin (83 citations) Penicillin (77 citations) Penicillin/streptomycin/amphotericin B solution (2 citations)

2 protocols using penicillin streptomycin solution

1

Aristolochic Acid-Induced HK-2 Cell Injury

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Human renal proximal tubular epithelial cells (HK-2 cells) were kindly provided by Shanghai Institute of Materia Medica (China) and cultured in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and 1.0% penicillin–streptomycin solution (Gemini Bio Products, West Sacramento, CA, USA).
Aristolochic acid I (AA I) (Purity HPLC ≥ 98%), Dimethyl sulfoxide (DMSO), Hoechst 33,258 were purchased from Solarbio (Solarbio Science & Technology Co., Ltd., Beijing, China); genipin (purity > 98%) was purchased from Sigma. The 3-(4,5-dimethyl thiazol-2-yl-)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) assay kit, lipid peroxidation (MDA) kit, and glutathione peroxidase (GSH-Px) were sourced from Nanjing Jiancheng Bioengineering Institute, Nanjing, China. Caspase-3 assay kit was purchased from Beyotime Biotechnology, Beijing, China. UCP2 polyclonal antibody (Cat # PA5-80203, Lot # AA2D12N), ECL solution and prestained protein marker were obtained from Thermo Fisher Scientific, Shanghai, China. To upregulate the expression of UCP2 in HK-2 cells, UCP2 DNA transfection using lipofectamine 2000 (Thermo Fisher Scientific, Shanghai, China) was performed.
Feng C., Anger E.E., Zhang X., Su S., Su C., Zhao S., Yu F, & Li J. (2022). Protective Effects of Mitochondrial Uncoupling Protein 2 against Aristolochic Acid I-Induced Toxicity in HK-2 Cells. International Journal of Molecular Sciences, 23(7), 3674.
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2

Embryonic Mouse Neuron Culture

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Cortices were dissected from E15 Swiss-Webster embryos in ice-cold HBSS (Thermo Fisher Scientific, 14175103) and dissociated with papain (Worthington Biochemical Corp, LS003126) and DNase I (Roche, 10104159001). Cells were resuspended in plating media (Neurobasal media (Thermo Fisher Scientific, 21103049), 1% Penicillin/Streptomycin Solution (Gemini Bio-Products, 400-109), 10% FBS) and filtered through a 100 μM cell strainer (VWR, 21008-950). Cell density was quantified using a Countess II Automated Cell Counter (Thermo Fisher Scientific, AMQAX1000), then plated on poly-D-Lysine-coated 12-well culture dishes at 0.5×106 cells or in 100mm culture dishes at 5×106 cells. Cultures were maintained in 5% CO2 at 37 °C in a cell culture incubator. After allowing four hours for the cells to adhere to the plate, the media was replaced and maintained with Neurobasal media supplemented with B-27 (Invitrogen, 17504-044), 1% Penicillin/Streptomycin, and 1% GlutaMAX Supplement (Thermo Fisher Scientific, 35050–079).
Cruz M.C., Sia R.A., Olson M., Cox G.M, & Heitman J. (2000). Comparison of the Roles of Calcineurin in Physiology and Virulence in Serotype D and Serotype A Strains of Cryptococcus neoformans. Infection and Immunity, 68(2), 982-985.
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