The protein was extracted from rat ovarian tissues by RIPA lysis buffer (Beijing Solarbio Science & Technology Co., Ltd.). Protein concentration was determined by a BCA protein assay kit according to the manufacturer's protocol and proteins (45 µg/lane) were separated via 10% SDS-PAGE and transferred onto PVDF membranes. The membranes were subsequently blocked with 5% BSA (Nanjing KeyGen Biotech Co., Ltd.) for 2 h at 4˚C, then incubated with Bcl-2 (1:1,000; cat. no. ab182858; Abcam), Bax (1:1000; cat. no. ab216985; Abcam), caspase-3 (1:1,000; cat. no. ab32150; Abcam) and GAPDH (1:1000; cat. no. ab8245; Abcam) rabbit anti-rat polyclonal antibodies overnight at 4˚C. Following the primary antibody incubation, the membrane was washed and incubated with a HRP-conjugated goat anti-rabbit secondary antibody (cat. no. BA1054; 1:2,000; Wuhan Boster Biological Technology, Ltd.) at 37˚C for 2 h. The blots were then incubated with WesternBright ECL (APG Bio, Ltd.). GAPDH was used as the internal reference. The results were analyzed using ImageJ version 1.47 (National Institutes of Health).
Bcl 2
Manufactured by Keygen Biotech
Sourced in China
Bcl-2 is a lab equipment product used for the detection and measurement of the Bcl-2 protein. Bcl-2 is a key regulator of apoptosis, or programmed cell death, and plays a crucial role in cell survival and proliferation. This product enables researchers to study the expression and function of Bcl-2 in various biological systems.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bcl2 associated x (bax), by Keygen Biotech (2 mentions)
Hrp conjugated goat anti rabbit secondary antibody, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Ripa lysis buffer, by Solarbio (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Caspase 3, by Abcam (1 mentions)
2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions)
Rpmi 1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
β actin, by Keygen Biotech (1 mentions)
Ab63929, by Abcam (1 mentions)
Kim 1, by Abcam (1 mentions)
Elx800, by Agilent Technologies (1 mentions)
Uv 2540, by Shimadzu (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine, by Merck Group (1 mentions)
Igfbp2, by Abcam (1 mentions)
Facscalibur, by BD (1 mentions)
Caspase3, by Keygen Biotech (1 mentions)
Gel doc xr, by Bio-Rad (1 mentions)
4 protocols using bcl 2
1
Rat Ovarian Protein Analysis Protocol
2
Synthesis and Evaluation of Iron-based Nanoparticles
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FeCl2·4H2O, FeCl3·6H2O, diethyleneglycol (DEG), Zn(NO3)2·6H2O, and NaOH were purchased from Shanghai Chemical Reagents Co (Shanghai, China). Dox was acquired from Hisun Phamaceuticals, Zhejiang, China. Cell Counting Kit-8 (CCK-8) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Sigma (St Louis, MO). RPMI-1640 culture medium, fetal bovine serum (FBS), and cultured plate were purchased from Gibco (Gibco/BRL, Carlsbad, CA). The antibodies of Bax, Bcl-2, Casepase 3, β-actin and horseradish peroxidase-conjugated IgG antibody were obtained from Nanjing KeyGen Biotech. Inc (Nanjing, China). All other reagents were of analytical grades.
3
Resveratrol Nanoparticles for Renal Protection
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Resveratrol (C14H12O3; > 98% pure) was purchased from Shaanxi Sciphar Biotechnology Co., Ltd. 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine and polyethylene glycol were purchased from Sigma Inc. Res-NPs were constructed as described in our previous study [12 (link)]. Phosphate-buffered saline (PBS), saline, apoptosis detection kit (Annexin VFITC/PI), protein extraction kit, malondialdehyde (MDA) kit, glutathione peroxidase (GSH-Px) kit, terminal deoxynucleotidyl transferase-mediated deoxyuridine-triphosphate nick end-labeling (TUNEL) apoptotic detection kit, and primary antibodies (BAX, caspase3, and BCL-2) were all purchased from Nanjing Key Gen Biotech. Co., Ltd. Blood urea nitrogen (BUN), C-reactive protein (CRP), cystatin C protease inhibitors (Cys-C), and serum creatinine (SCr) were all obtained from Weiteman, Co., Ltd., (Nanjing, China).
The following instruments were used: automatic biochemical analyzer (Hitachi 7100, Japan), microplate reader (ELx800, BioTek, USA), flow cytometer (FACSCalibur, Becton-Dickinson, USA), inverted fluorescence microscope (IX51, Olympus, Japan), Western electrophoresis apparatus (164-5051, Bio-Rad, USA), spectrophotometer (UV-2540, Shimadzu, Japan), and gel imager (Gel Doc XR, Bio-Rad, USA). Primary antibodies (IGFBP2, AAab136494; KIM-1, ab47634; NGAL, and ab63929) were purchased from Abcam (Cambridge, UK).
The following instruments were used: automatic biochemical analyzer (Hitachi 7100, Japan), microplate reader (ELx800, BioTek, USA), flow cytometer (FACSCalibur, Becton-Dickinson, USA), inverted fluorescence microscope (IX51, Olympus, Japan), Western electrophoresis apparatus (164-5051, Bio-Rad, USA), spectrophotometer (UV-2540, Shimadzu, Japan), and gel imager (Gel Doc XR, Bio-Rad, USA). Primary antibodies (IGFBP2, AAab136494; KIM-1, ab47634; NGAL, and ab63929) were purchased from Abcam (Cambridge, UK).
4
Neuroprotective Potential of Natural Compounds
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Ringer's solution, containing 122 mM sodium chloride, 3 mM potassium chloride, 0.4 mM monopotassium phosphate, 1.2 mM magnesium sulfate, 25 mM sodium bicarbonate, and 1.2 mM calcium chloride, was purchased from Millipore (MA, USA). Pachymic acid, liquiritin, rhynchophylline, isorhynchophylline, corynoxeine, and isocorynoxeine (purity ≥ 99%) were purchased from National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) was purchased from Gibco/BRL (Grand Island, NY, USA). Methyl thiazolyl tetrazolium (MTT), dimethyl sulfoxide (DMSO), and trypsin were provided by Sigma Chemical (St. Louis, MO, USA). Aβ1–42 (purity ≥ 95%) was purchased from Abcam (Cambridge, USA). HPLC-grade acetonitrile was purchased from Thermo Fisher Scientific (Massachusetts, USA). EliVision plus and DAB kits were provided by Fuzhou Maixin Biotech. Co., Ltd. (Fuzhou, China). Bax, Bcl-2, caspase-3 and caspase-9 antibodies, annexin V-FITC/PI assay kit, basal DMEM medium, cell lysis solution, and 0.1% DEPC water were purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). Nimodipine was provided by Bayer healthcare Co., Ltd. (Leverkusen, Germany).
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