Patients underwent LESS salpingectomy and hysterectomy using a single umbilical incision, as described previously.7 (link) Briefly, the procedure was conducted as follows. A patient was placed in the lithotomy position. A single-port platform was positioned, followed by inflation with CO2. Salpingectomy was performed at the most proximal part of the fallopian tube (Figure 2A ). Additional thermal cauterization (AD) of one of the uterine cornu was performed using a vessel sealing device for 10 seconds after the removal of the fallopian tube, and no additional cauterization (NO) was applied to the other side. We grasped the cutting edge of the cornu with the jaw of a LigaSure (Covidien, Boulder, CO, US) (Figure 2B ). Hysterectomy was performed, and the uterus was subsequently removed through the vagina, followed by closure of the vaginal stump and the abdominal skin incision. Three tissue samples were collected from each cornu. Tissue samples were numbered 1–6 (Figure 2C ); lateral sections of the tissues were designated 1and 6, intermediate sections of the tissues were designated 2 and 5, and medial sections of the tissues were designated 3 and 4.
A LigaSure 5-mm blunt tip laparoscopic instrument (LigaSure; LF1537, Covidien, Mansfield, MA, USA) was used for salpingectomy and additional cauterization.
A LigaSure 5-mm blunt tip laparoscopic instrument (LigaSure; LF1537, Covidien, Mansfield, MA, USA) was used for salpingectomy and additional cauterization.