Hcc1937

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

The HCC1937 is a cultured human cell line derived from a breast carcinoma. It serves as a tool for research and analysis in the field of cell biology and cancer studies.

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Lab products found in correlation

Mda mb 231, by National Collection of Authenticated Cell Cultures (16 mentions) Mcf 7, by National Collection of Authenticated Cell Cultures (11 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions) Bt 549, by National Collection of Authenticated Cell Cultures (5 mentions) Hcc1937, by Thermo Fisher Scientific (4 mentions) Mda mb 453, by National Collection of Authenticated Cell Cultures (4 mentions) Mcf 10a, by National Collection of Authenticated Cell Cultures (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Mda mb 231, by Thermo Fisher Scientific (3 mentions) Mcf 7, by Thermo Fisher Scientific (3 mentions) Hs578t, by National Collection of Authenticated Cell Cultures (3 mentions) Erk1 2, by Cell Signaling Technology (2 mentions) P erk1 2, by Cell Signaling Technology (2 mentions) Gapdh antibody, by Santa Cruz Biotechnology (2 mentions) Nfκb 65, by Cell Signaling Technology (2 mentions) β catenin, by Cell Signaling Technology (2 mentions) P nfκb 65, by Cell Signaling Technology (2 mentions) P akt1, by Cell Signaling Technology (2 mentions) P β catenin, by Cell Signaling Technology (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)

19 protocols using hcc1937

Epithelial-Mesenchymal Transition Mechanism

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HCC1937 and MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Genview (Campbellfield, VIC, Australia); SYBR Master Mixture was purchased from Takara (Shimogyo-ku, Kyoto, Japan); antibodies against CCT3, CDH1, Slug, Snail, VIM, mTOR, ERK1/2, p-ERK1/2, p-AKT1, P38, p-P38, NFκB-65, p-NFκB-65, β-catenin and p-β-catenin were purchased from Cell Signaling Technology (Danvers, MA, USA); and antibodies against CDH2, MMP2, FN1, MYC, p-mTOR and AKT1 were purchased from Abcam (Cambridge, MA, USA). GAPDH antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Xu G., Bu S., Wang X., Zhang H, & Ge H. (2020). Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells. Cancer Cell International, 20, 218.

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Breast Cancer Cell Line Characterization

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Cells and materials HCC1937 and MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Genview (Campbell eld, VIC, Australia); SYBR Master Mixture was purchased from Takara (Shimogyo-ku, Kyoto, Japan); antibodies against CCT3, CDH1, Slug, Snail, VIM, mTOR, ERK1/2, p-ERK1/2, p-AKT1, P38, p-P38, NFκB-65, p-NFκB-65, β-catenin and p-β-catenin were purchased from Cell Signaling Technology (Danvers, MA, USA); and antibodies against CDH2, MMP2, FN1, MYC, p-mTOR and AKT1 were purchased from Abcam (Cambridge, MA, USA). GAPDH antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).
Cell culture HCC1937 and MDA-MB-231 cell lines were cultured in RPMI 1640 medium(Gibco; Gaithersburg, MD, United States) supplemented with 10% foetal bovine serum. The cells were maintained at 37°C in a 5% CO 2 humidi ed incubator.

Xu G., Bu S., Wang X., Zhang H., & Ge H. (2020). Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells.

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Culturing Breast Cell Lines for Research

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The normal breast cell line, MCF10A, and the human breast cancer cell lines, MDA-MB-231, HCC1937, ZR-75-1 and MCF7, were purchased from Cell Bank of the Chinese Academy of Science (Shanghai, China). MCF10A cells were cultured in mammary epithelial cell growth (MEGM) (Gibco BRL. Co. Ltd., Grand Island, NY, USA), MDA-MB-231 cells were cultured in L-15 medium (Gibco BRL. Co. Ltd.), HCC1937 and ZR-75-1 cells were cultured in RPMI 1640 (Gibco BRL. Co. Ltd.), MCF7 cells were cultured in minimum eagle medium (MEM) (Gibco BRL. Co. Ltd.), supplemented with FBS (Gibco BRL. Co. Ltd.) and 1% penicillin–streptomycin (Beyotime Biotech. Shanghai, China) in a humidified incubator at 37 ℃ with 5% CO₂.
This study was approved by the Ethics Committee of Jinshan Hospital, Fudan University, Shanghai, China.

Gu J., Xu T., Huang Q.H., Zhang C.M, & Chen H.Y. (2019). HMGB3 silence inhibits breast cancer cell proliferation and tumor growth by interacting with hypoxia-inducible factor 1α. Cancer Management and Research, 11, 5075-5089.

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Breast Cancer Cell Line Maintenance and Treatment

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The breast cancer cell lines HCC-1937, MCF-7, MCF-7/ADR, MDA-MB-231 and MDA-MB-453 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were grown in 25 cm² cell culture flasks and maintained in Dulbecco’s-modified Eagle’s medium (DMEM, HyClone) or RPMI 1640 medium (HyClone) supplemented with 10% foetal bovine serum, 100 U/ml penicillin G, and 100 U/ml streptomycin at 37 °C in 5% CO2 and 95% air as previously described [9 (link)]. Dox was purchased from Yuanye Biotech (Shanghai, China) and diluted in PBS. rhPTN was purchased from PeproTech (London, UK), and cells were treated with rhPTN (5 μg/mL) for 20 min according to the manufacturer’s instructions.

Huang P., Ouyang D.J., Chang S., Li M.Y., Li L., Li Q.Y., Zeng R., Zou Q.Y., Su J., Zhao P., Pei L, & Yi W.J. (2018). Chemotherapy-driven increases in the CDKN1A/PTN/PTPRZ1 axis promote chemoresistance by activating the NF-κB pathway in breast cancer cells. Cell Communication and Signaling : CCS, 16, 92.

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Breast Cancer Cell Line Characterization

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Wild-type mouse breast cancer cell lines 4TO7, 4T1, and EO771 and a stable EO771 cell line overexpressing firefly luciferase (EO771FL) were kindly provided by Dr. Ralph A. Reisfeld (The Scripps Research Institute, CA, USA). Mouse breast cancer cell line EMT6 was obtained from the Laboratory Animal Research Center of the Fourth Military Medical University (Xi'an, Shanxi, China). Human breast epithelial cell lines MCF 10A and human breast cancer cell lines MDA-MB-231, HCC1937, MDA-MB-453, MDA-MB-436, T-47D, MCF-7, and ZR-75-1 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China); these cell lines were authenticated by short-tandem repeat profiling. The MDA-MB-231 and 4T1 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS), 100 μg/mL streptomycin, and 100 U/mL penicillin. The T-47D cells were maintained in DMEM containing 10% FBS, 1% non-essential amino acids, 100 μg/mL streptomycin and 100 U/mL penicillin. EO771FL cells were maintained in RPMI-1640 medium supplemented with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin. All cells were cultured in an incubator at 37 °C with 5% CO₂.

Gao W., Wen H., Liang L., Dong X., Du R., Zhou W., Zhang X., Zhang C., Xiang R, & Li N. (2021). IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer. Theranostics, 11(6), 2564-2580.

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Ethical Breast Cancer Sample Extraction

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This study was conducted in accordance with the principles stated in the Declaration of Helsinki (as revised in 2013). Ethical clearance was granted from the First Affiliated Hospital of Nanjing Medical University Medical Science Research Ethics Committee (Ethics code: 2021-SR-308). Breast cancer specimens were harvested from the Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, and written informed consent was provided by the patients or their next of kin. Surgical specimens were resected, snap-frozen in liquid nitrogen, and deposited in the Biological Sample Bank of Jiangsu Province Hospital. The catalogue number of samples used in this study were 399, 459, 461, 474, 476, 477 and 488. The breast tumor cells MCF-7, ZR-75-1, BT-474, SK-BR-3, MDA-MB-453, MDA-MB-231, BT-549, Hs-578T, HCC1806, and HCC1937 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). For western blot analysis, frozen human breast cancer samples were ground and homogenized in a mortar and pestle under liquid nitrogen and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Solarbio, Beijing, China) supplemented with protease inhibitors phenylmethylsulfonyl fluoride (PMSF; Beyotime, Shanghai, China). Cells were rinsed twice with phosphate-buffered saline (PBS) and lysed in RIPA buffer on ice.

Fang Z., Song Y.X., Wo G.Q., Zhou H.L., Li L., Yang S.Y., Chen X., Zhang J, & Tang J.H. (2022). Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer. Annals of Translational Medicine, 10(23), 1280.

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Culturing Breast Cell Lines

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BC cell lines (HCC1937, BT-20, MCF-7, and MDB-MB-436) and a human normal breast cell line (MCF-10A) were commercially obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were incubated in Dulbecco's Modi ed Eagle Medium (DMEM, Gibco, USA) supplemented with 10% FBS at 37℃ in the presence of 5% CO 2 .

Jia H., Wu D., Zhang Z., & Li S. (2020). Sp1-activated Long Noncoding RNA NCK1-AS1 Facilitates Cell Growth of Breast Cancer via Sponging miR-361-5p .

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TNBC Cell Line Manipulation and Lentiviral Transduction

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The human TNBC cell lines MDA-MB-231, BT-549, HCC1937, and Hs578T were purchased from the Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. Cell were cultured in Dulbecco's Modified Eagle Medium (DMEM; HyClone, Logan, USA), supplemented with 10% fetal bovine serum (FBS; Gibco, Carlsbad, USA) and 1% penicillin and streptomycin solution and incubated at 37 °C and 5% CO₂. The short hairpin RNA (shRNA) targeting YTHDF3 was subcloned into the GV493 lentiviral shRNA vector (Genechem, Shanghai, China). For overexpression of ZEB1, the construct was generated by subcloning PCR amplified full-length human ZEB1 cDNA into the vector. The constructed lentiviral vectors were packaged into viruses in 293 T cells which were then harvested and the concentrated viruses were added to TNBC cells and cultured for 72 hours. Gene sequences are provided in Table S1.

Lin Y., Jin X., Nie Q., Chen M., Guo W., Chen L., Li Y., Chen X., Zhang W., Chen H., Jiang M., Xiao H., Zhang J., Fu F, & Wang C. (2022). YTHDF3 facilitates triple-negative breast cancer progression and metastasis by stabilizing ZEB1 mRNA in an m6A-dependent manner. Annals of Translational Medicine, 10(2), 83.

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TNBC Cell Line Culture Protocol

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The TNBC cell lines MDA-MB-231 and HCC1937 were acquired from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). MDA-MB-231 and HCC1937 cells were cultured in DMEM (Gibco, Grand Island, NY, USA) and RPMI-1640 (Gibco) media, respectively. Both media were supplemented with 10% fetal bovine serum (GIBCO-BRL, Invitrogen, USA), 100 ug/mL penicillin and 100 U/mL streptomycin (Shanghai Genebase Gen-Tech, Shanghai, China) The cells were maintained at 37°C in a 5% CO2 incubator.

Ni K., Huang Z., Zhu Y., Xue D., Jin Q., Zhang C, & Gu C. (2021). The lncRNA ADAMTS9-AS2 Regulates RPL22 to Modulate TNBC Progression via Controlling the TGF-β Signaling Pathway. Frontiers in Oncology, 11, 654472.

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Culturing Human Breast Cancer Cells

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Human breast cancer cell lines, HCC1937 and MCF7, were purchased from the Cell Bank of Type Culture Collection of the Chinese Academy of Sciences. Cells were maintained in RPMI-1640 medium supplemented with 10% FBS (both purchased from Corning, Inc.), at 37˚C with 95% air and 5% CO₂.

Li H., Zhang H., Tan X., Liu D., Guo R., Wang M., Tang Y., Zheng K., Chen W., Li H., Tan M., Wang K., Liu R, & Tang S. (2021). Overexpression of ING3 is associated with attenuation of migration and invasion in breast cancer. Experimental and Therapeutic Medicine, 22(1), 699.

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