Nci h292 human airway epithelial cells

Primary nasal epithelial cells were obtained by digesting nasal turbinates of non-allergic patients with 0.5 mg/ml collagenase 4 (Worthington Biochemical Corp., Lakewood, NJ) for 1 h in Hanks’ balanced salt solution (HBSS; Sigma-Aldrich, Zwijndrecht, The Netherlands). Subsequently, epithelial cells were isolated by magnetic activated cell sorting (MACS), according to the manufacturers instruction (Miltenyi Biotec, Leiden, The Netherlands), resuspended in bronchial epithelial growth medium (BEGM) (Lonza Clonetics, Breda, The Netherlands), and seeded in a 75 ml flask. Culture medium was replaced every other day. Cells were grown to 80 % confluency in fully humidified air containing 5 % CO₂ at 37 °C.
NCI-H292 human airway epithelial cells (American Type Culture Collection, Mannassas, VA, USA) were cultured in RPMI 1640 medium (Invitrogen, Breda, The Netherland) supplemented with 1.25 mM l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 10 % (v/v) fetal bovine serum (HyClone, Logan, UT, USA). Cells were grown in fully humidified air with 5 % CO₂ at 37 °C and sub cultured weekly.

NCI-H292 human airway epithelial cells (American Type Culture Collection, USA) were cultured in RPMI 1640 culture medium (Invitrogen, NL) supplemented with 10% (v/v) fetal calf serum (HyClone, USA), 1.25 mM of glutamine, 100 U/mL of penicillin and 100 µg/mL of streptomycin. Cells were grown in fully humidified air containing 5% of CO₂ at 37°C.