All solvents for HPLC-DAD-MS analyses (HPLC grade), formic acid, and epigallocatechin gallate (analytical grade) were purchased from Sigma Aldrich Chemical Company Inc. (Milwaukee, WI, USA). Tyrosol, gallic acid, ellagic acid, catechin, and malvidin 3-O-glucoside were supplied by Extrasynthèse S.A. (Lyon, Nord-Genay, France). HydroxyTyrosol was synthetized in the laboratory [80 (link)]. HPLC-grade water was obtained via distillation and purification with a Labconco Water Pro PS polishing station (Labconco Corporation, Kansas City, MO, USA). Potassium sorbate, benzoic acid, potassium hydroxide, and hydrochloric acid 37% were purchased from Carlo Erba Reagents Srl (Cornaredo, Milan, Italy), Tween 20 from Biolife Italiana Srl (Milan, Italy), and Potato Dextrose Agar from Oxoid Ltd. (Basingstoke, Hampshire, UK). Sterile plasticware and cotton swabs were purchased from Unifo Srl (Zero Branco, Treviso, Italy).
Tween 20
Manufactured by Biolife
Sourced in Italy
Tween 20 is a non-ionic detergent commonly used in various laboratory applications. It is a polyoxyethylene sorbitan monolaurate compound that serves as a surfactant, emulsifier, and solubilizing agent.
Automatically generated - may contain errors
Lab products found in correlation
Tyrosol, by Extrasynthese (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Ellagic acid, by Extrasynthese (1 mentions)
Catechin, by Extrasynthese (1 mentions)
Gallic acid, by Extrasynthese (1 mentions)
Potato dextrose agar (pda), by Thermo Fisher Scientific (1 mentions)
Hplc dad ms, by Merck Group (1 mentions)
Hplc grade formic acid, by Merck Group (1 mentions)
Potassium hydroxide, by Carlo Erba (1 mentions)
Hydrochloric acid (hcl), by Carlo Erba (1 mentions)
Water pro ps polishing station, by Labconco (1 mentions)
Malvidin 3 o glucoside, by Extrasynthese (1 mentions)
Potassium sorbate, by Carlo Erba (1 mentions)
Benzoic acid, by Carlo Erba (1 mentions)
2 protocols using tween 20
1
HPLC-DAD-MS Analysis of Bioactive Compounds
2
Determining Antimicrobial Efficacy of BEOs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BEOs with an antimicrobial activity from moderate to strong were tested for minimal inhibitory concentration (MIC) according to a modified NCCLS/CLSI standard method (NCCLS/CLSI, 2015 ). Serial two-fold dilutions of each BEO were made in a concentration ranging from 5 to 0.31 μL/mL in 10 mL sterile test tubes containing trypticase soy broth with 0.6% yeast extract. At this solution 5% (v/v) Tween-20 (Biolife) was incorporated into the broth medium to enhance oil solubility. The inoculums were prepared from overnight broth cultures of sensitive strains (logarithmic growth phase cells). A 400-pL suspension of tested microorganisms was added to each tube. For positive and negative control we used two broth tubes containing respectively 50 pg/mL of Streptomycin and only microorganism inoculums. MIC was assumed as the concentration in the lowest serial dilution of the BEOs that resulted in the lack of visible microorganism growth in tubes after 24 h incubation.
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