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Detoxified bsa

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Detoxified BSA is a high-quality bovine serum albumin (BSA) product that has undergone a proprietary detoxification process. It is suitable for use in various cell culture and protein-based applications where a pure, endotoxin-low BSA is required.

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Lab products found in correlation

Holo transferrin, by Merck Group (3 mentions) Dexamethasone (dex), by Merck Group (3 mentions) Erythropoietin, by Thermo Fisher Scientific (2 mentions) Human insulin, by Merck Group (2 mentions) Automacs pro, by Miltenyi Biotec (2 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (2 mentions) Biotin conjugated lineage antibodies, by BD (2 mentions) Biocoat cellware, by Corning (2 mentions) Erythropoietin, by Amgen (1 mentions) Recombinant human insulin, by Merck Group (1 mentions) Epoetin epo, by Amgen (1 mentions) Recombinant mouse igf 1, by R&D Systems (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Stemspan sfem, by STEMCELL (1 mentions) Fetal bovine serum (fbs), by STEMCELL (1 mentions)

3 protocols using detoxified bsa

1

Erythroid Differentiation of Sf3b1 Mutant Cells

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Bone marrow cells that were isolated from Sf3b1K700E +/- mice and littermate controls were lineage depleted using the Lineage Cell Depletion Kit, mouse (Miltenyi Biotech) according to manufacturer’s protocols. Lineage negative cells were immediately transduced with lentiviral shRNAs targeting SF3A2 or controls (MOI −90) by spinfection at 2000 rpm for 90 min. The cells were cultured in erythroid maintenance medium (StemSpan-SFEM; StemCell Technologies) supplemented with 100 ng/mL recombinant mouse stem cell factor (SCF) (R&D Systems), 40 ng/mL recombinant mouse IGF1 (R&D Systems), 100 nM dexamethasone (Sigma), and 2 U/mL erythropoietin (Amgen) and cultured at 37°C for 36 hr. Following this, the cells were cultured for another 48 hr in erythroid differentiation medium (Iscove modified Dulbecco’s medium containing 15% (vol/vol) FBS (Stemcell), 1% detoxified BSA (Stemcell), 500 μg/mL holo-transferrin (Sigma-Aldrich), 0.5 U/mL Epoetin (Epo; Amgen), 10 μg/mL recombinant human insulin (Sigma-Aldrich), and 2 mM L-glutamine (Invitrogen)) at 37°C.
Nandakumar S.K., McFarland S.K., Mateyka L.M., Lareau C.A., Ulirsch J.C., Ludwig L.S., Agarwal G., Engreitz J.M., Przychodzen B., McConkey M., Cowley G.S., Doench J.G., Maciejewski J.P., Ebert B.L., Root D.E, & Sankaran V.G. (2019). Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis. eLife, 8, e44080.
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2

Erythropoiesis from Lineage-Depleted BM Cells

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Whole BM cells were labeled with biotin-conjugated lineage antibodies (cocktail of anti-CD3ε, anti-CD11b, anti-CD45R/B220, anti-Gr1, anti-CD5, and anti-TER-119) (BD Pharmingen) and purified using anti-biotin beads and negative selection on the AutoMACS Pro (Miltenyi). Purified cells were then seeded in fibronectin-coated (2 μg/cm2) tissue-culture treated polystyrene wells (Corning® BioCoat™ Cellware) at a cell density of 105/mL. Erythroid differentiation was carried out according to modified published protocols57 . The erythropoietic medium was IMDM supplemented with erythropoietin at 10 U/mL, 10 ng/mL stem cell factor SCF, PeproTech), 10 μM Dexamethasone (Sigma-Aldrich), 15% FBS, 1% detoxified BSA (StemCell Technologies), 200 μg/mL holotransferrin (Sigma-Aldrich), 10 mg/mL human insulin (Sigma-Aldrich), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, and penicillin-streptomycin. After 48 h, the medium was replaced by IMDM medium containing 20% FBS, 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, and penicillin-streptomycin. 50% of the culture media was replaced after 48 h and cell density was maintained at 0.5 × 106/mL. Total culture period for the assay was 6 days. Recombinant mouse IL-22 (PeproTech/Cell Signaling) was used where indicated. RII-RIV populations were gated as shown in Extended Data Fig. 2a.
Raundhal M., Ghosh S., Myers S.A., Cuoco M.S., Singer M., Carr S.A., Waikar S.S., Bonventre J.V., Ritz J., Stone R.M., Steensma D.P., Regev A, & Glimcher L.H. (2021). Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias. Nature immunology, 22(4), 520-529.
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3

Erythropoiesis from Lineage-Depleted BM Cells

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Whole BM cells were labeled with biotin-conjugated lineage antibodies (cocktail of anti-CD3ε, anti-CD11b, anti-CD45R/B220, anti-Gr1, anti-CD5, and anti-TER-119) (BD Pharmingen) and purified using anti-biotin beads and negative selection on the AutoMACS Pro (Miltenyi). Purified cells were then seeded in fibronectin-coated (2 μg/cm2) tissue-culture treated polystyrene wells (Corning® BioCoat™ Cellware) at a cell density of 105/mL. Erythroid differentiation was carried out according to modified published protocols57 . The erythropoietic medium was IMDM supplemented with erythropoietin at 10 U/mL, 10 ng/mL stem cell factor SCF, PeproTech), 10 μM Dexamethasone (Sigma-Aldrich), 15% FBS, 1% detoxified BSA (StemCell Technologies), 200 μg/mL holotransferrin (Sigma-Aldrich), 10 mg/mL human insulin (Sigma-Aldrich), 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, and penicillin-streptomycin. After 48 h, the medium was replaced by IMDM medium containing 20% FBS, 2 mM L-glutamine, 0.1 mM β-mercaptoethanol, and penicillin-streptomycin. 50% of the culture media was replaced after 48 h and cell density was maintained at 0.5 × 106/mL. Total culture period for the assay was 6 days. Recombinant mouse IL-22 (PeproTech/Cell Signaling) was used where indicated. RII-RIV populations were gated as shown in Extended Data Fig. 2a.
Raundhal M., Ghosh S., Myers S.A., Cuoco M.S., Singer M., Carr S.A., Waikar S.S., Bonventre J.V., Ritz J., Stone R.M., Steensma D.P., Regev A, & Glimcher L.H. (2021). Blockade of IL-22 signaling reverses erythroid dysfunction in stress-induced anemias. Nature immunology, 22(4), 520-529.
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