N acetyl l tyrosine

Human serum albumin (HSA), essentially fatty acid free (A1887), phenylbutazone, taurine, N-acetyl-l-tryptophan, N-acetyl-l-tyrosine, methionine, cysteine, phenylalanine, alanine, serine, arginine, leucine, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), and 2,4-dinitrophenylhydrazine were purchased from Sigma-Aldrich (St. Louis, MO, USA). HSA was dissolved in 10 mmol·L⁻¹ phosphate-buffered saline (PBS) at pH 7.4 to give a 1 mmol·L⁻¹ stock solution, and its concentration was measured by its absorbance (ε_{280 nm} = 35,219 mol⁻¹·L·cm⁻¹). Hypochlorous acid (HOCl) was prepared by diluting a 5% stock solution, and the concentration was determined spectrophotometrically after dilution in 0.01 mol·L⁻¹ NaOH, pH 12 (ε_{292 nm} = 350 mol⁻¹·L·cm⁻¹). taurine monochloramine (Tau-NHCl) was prepared by the addition of 5 mmol·L⁻¹ HOCl to 50 mmol·L⁻¹ taurine in PBS at pH 7.4 [53 (link)]. Hypobromous acid (HOBr) was synthesized by combining 100 mmol·L⁻¹ HOCl and 200 mmol·L⁻¹ NaBr in water. taurine dibromamine (Tau-NBr₂) was prepared by the addition of 5 mmol·L⁻¹ HOBr to 2.5 mmol·L⁻¹ taurine in PBS at pH 7.4 [54 (link)]. A Perkin Elmer Lambda 35 UV–visible (UV–Vis) spectrophotometer (Shelton, CT, USA) was used for the UV–Vis measurements.