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Polyclonal anti myc a 14

Manufactured by Merck Group
Sourced in United States

Polyclonal anti-myc (A-14) is a laboratory reagent produced by Merck Group. It is an antibody that recognizes the c-myc epitope. The core function of this product is to enable the detection and analysis of c-myc tagged proteins in various experimental applications.

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3 protocols using polyclonal anti myc a 14

1

Cell Culture Reagents Protocol

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𝛼-MEM (modified Eagle’s medium), fetal bovine serum, and all other tissue culture reagents were purchased from biological industries (Beit Haemek, Israel). Horseradish-peroxidase (HRP-) -conjugated goat anti-rabbit antibodies were obtained from Promega (Madison, WI, USA). Polyclonal anti-myc (A-14) and other standard chemicals were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
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2

Protein Expression Quantification

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All tissue culture reagents including fetal bovine serum and standard culture medium α-MEM (modified Eagle's medium) were purchased from Biological Industries (Beit Haemek, Israel). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibodies were obtained from Promega (Madison, WI, USA). Polyclonal anti-myc (A-14) and other standard chemicals were purchased from Sigma.
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3

L6-GLUT4myc Cell Line Protocol

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Materials: The rat L6 muscle cell line, stably expressing myc-tagged GLUT4 (L6-GLUT4myc cells), was obtained from Kerafast (Boston, MA, USA). Fetal bovine serum, modified Eagle’s medium (α-MEM), standard culture medium, and all other tissue culture reagents were purchased from Biological Industries (Beit Haemek, Israel). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibodies were obtained from Promega (Madison, WI, USA). Polyclonal anti-myc (A-14); the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent; methoxyamine hydrochloride; pyridine; N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA); and other standard chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA).
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