The following antibodies were used: allophycocyanin (APC)-H7–conjugated anti-CD3 (clone SK7), FITC-conjugated anti-CD4 (clone RPA-T4), APC-conjugated anti-CD4 (clone RPA-T4), Pacific blue (PB)–conjugated anti-CD4 (clone RPA-T4), APC-H7–conjugated anti-CD8 (clone SK1), PerCP-Cy5.5–conjugated anti-CD8 (clone SK1), PerCP-conjugated anti-CD69 (clone L78), PECy7-conjugated anti-CD279 (PD-1; clone EH12.1), APC-conjugated anti-TNF (MAbII), FITC-conjugated anti-CD25 (OX-39), PECy-conjugated anti-TNF (clone MabII; BD); energy coupled dye (ECD)–conjugated anti-CD3 (clone UCHT1), ECD-conjugated anti-CD45RA (clone 2H4), ECD-conjugated anti-CD4 (clone T4; Beckman Coulter); PECy5.5-conjugated anti-2B4 (CD244; clone C1.7), PE-conjugated anti-SLAM (CD150; clone A12), PB-conjugated anti-CD57 (clone HCD57), Alexa Fluor 647–conjugated anti-CD160 (clone BY55; BioLegend); EFluor 625NC-conjugated anti-CD8 (clone RPA-T8; eBioscience); and FITC-conjugated anti-CCR7 (clone 150503) and Alexa Fluor 700–conjugated anti–HLA-DR (clone LN3; R&D Systems).
Percp cy5.5 conjugated anti cd8 clone sk1
Manufactured by BD
Sourced in United States
PerCP-Cy5.5–conjugated anti-CD8 (clone SK1) is a fluorescently labeled antibody that binds to the CD8 marker on T cells. It is used for the identification and enumeration of CD8-positive cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Fitc conjugated anti ccr7 clone 150503, by R&D Systems (1 mentions)
Apc conjugated anti tnf, by BD (1 mentions)
Ecd conjugated anti cd3, by Beckman Coulter (1 mentions)
Apc h7 conjugated anti cd8, by BD (1 mentions)
Apc h7 conjugated anti cd3 clone sk7, by BD (1 mentions)
Anti cd28 clone cd28, by BD (1 mentions)
2 protocols using percp cy5.5 conjugated anti cd8 clone sk1
1
Multicolor Flow Cytometry Panel
2
Comprehensive Immunophenotyping of Activated T Cells
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The following antibodies were used. Allophycocyanin (APC)-H7-conjugated anti-CD3 (clone SK7), peridinin chlorophyll protein (PerCP)-Cy5.5-conjugated anti-CD8 (clone SK1), Pacific Blue (PB)-, fluorescein isothiocyanate (FITC)-, or phycoerythrin (PE)-CF594-conjugated anti-CD4 (clone RPA-T4), V450-conjugated anti-HLA-DR (clone G46-6), PE-Cy7-conjugated anti-CD25 (clone M-A251), PerCP-Cy5.5-conjugated anti-CD69 (clone L78), purified coating anti-CD3 (clone UCHT1), and anti-CD28 (clone CD28.2) monoclonal antibodies (MAbs) were purchased from BD (Becton Dickinson, USA) or from Biolegend (Switzerland). PE-conjugated anti-acetyl H3 and H4 were from Merck Millipore (USA), and electron-coupled dye (ECD)-conjugated anti-CD45RA (clone 2H4) was from Beckman Coulter (USA).
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