Anti f4 80

Manufactured by Agilent Technologies

Anti-F4/80 is a laboratory tool used for the detection and identification of F4/80-positive cells, which are a type of macrophage commonly found in various tissues. This product is designed to assist researchers in their investigations of immune system function and disease pathogenesis.

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Lab products found in correlation

Anti cd3, by Agilent Technologies (2 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Dako autostainer plus, by Agilent Technologies (1 mentions) Anti foxp3, by Agilent Technologies (1 mentions) Target retrieval solution, by Agilent Technologies (1 mentions) Anti foxp3, by Thermo Fisher Scientific (1 mentions) Anti f4 80, by Bio-Rad (1 mentions) Anti cd45, by Agilent Technologies (1 mentions)

2 protocols using anti f4 80

Immunohistochemical Analysis of Lung Markers

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Paraffin embedded lungs were sectioned, deparaffinized, hydrated in deionized water, and treated with Target Retrieval Solution (DAKO, Carpinteria, CA) at either pH 9 (anti-CD3, anti-Foxp3, anti-Ly6C/Ly6G) or pH 6 (anti-F4/80). All slides were then stained using a DAKO Autostainer Plus (DAKO, Carpinteria, CA) with the standard labeled streptavidin-biotin protocol. Lung sections were stained at room temperature for 60 min with polyclonal rabbit anti-CD3 (DAKO; 1:100), anti-F4/80 (AbD Serotec; clone A3-1; 1:250), anti-Foxp3 (eBioscience, clone FJK-16s; 1:25) or anti-Ly6C/Ly6G (clone NIMP-R14; 1:250). Negative controls were performed with omission of the primary antibody. Slides were then developed with Tertiary Streptavidin-HRP (DAKO) and counterstained with hematoxylin.

Reeme A.E, & Robinson R.T. (2016). Dietary Vitamin D3 suppresses pulmonary immunopathology associated with late stage tuberculosis in C3HeB/FeJ mice. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1293-1304.

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Antibody Specificity for Signaling Proteins

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Antibodies to total ERK1/2, active phosphorylated ERK1/2 (Thr202/Tyr204; P-ERK1/2), and to total JNK1/2, phospho-NFκB1/p105 (Ser933; P-p105), phospho-STAT3 (Tyr705; P-STAT3) and active phospho-p38MAPK (Thr180-Tyr182; P-p38) were from Cell Signaling Technology. Anti-P-p38 antibody recognized all phosphorylated p38 isoforms, since the phosphorylation sites of all four p38MAPK are very similar, there are not specific phospho-p38 antibodies for each p38MAPK isoform. Anti-STAT3 and -p38α were from Santa Cruz, anti-active phospho-JNK1/2 (Thr183-Tyr185; P-JNK) from Biosource, anti-BrdU and -myeloperoxidase (MPO) from Abcam, and anti-p38γ and -p38δ antibodies were raised and purified as described [49 (link), 50 (link)]. Anti-CD3, anti-F4/80, anti-CD45, anti-γδ TCR and anti-Ly6G were from Dako Cytomation.

Zur R., Garcia-Ibanez L., Nunez-Buiza A., Aparicio N., Liappas G., Escós A., Risco A., Page A., Saiz-Ladera C., Alsina-Beauchamp D., Montans J., Paramio J.M, & Cuenda A. (2015). Combined deletion of p38γ and p38δ reduces skin inflammation and protects from carcinogenesis. Oncotarget, 6(15), 12920-12935.

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