β-glucosidase activity was determined using pNPGlu as substrate. At least three technical replicates were performed for these experiments. The assay mixture (1 mL) consisting of 2 mM pNPGlu in 50 mM sodium citrate buffer (pH 6.0) was incubated with the enzyme at 45 °C for 10 min. The reaction was stopped by adding 1 mL of 1 M Na2CO3 and the amount of p-nitrophenol released was determined by measuring the absorbance at 400 nm in a JASCO V-650 UV–VIS spectrophotometer running Spectra Manager Version 2 (Jasco, Tokyo, Japan). One unit of enzyme activity was defined as the amount of enzyme releasing 1 μmol of pNP per minute under the above-mentioned assay conditions. Specific activity was expressed in units of active enzyme per milligram of protein.
V 650 uv vis
Manufactured by Jasco
Sourced in Japan
The V-650 UV–VIS is a spectrophotometer designed for ultraviolet and visible light absorption and transmission measurements. It is capable of performing various spectroscopic analyses, including wavelength scanning, quantitative determination, and kinetic studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using v 650 uv vis
1
Quantifying β-Glucosidase Activity Using pNPGlu
2
Quantification of Brimonidine Tartrate by UV-Vis Spectrophotometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brimonidine tartrate quantification was done based on the spectrometric method developed by Bhagav et al. 21 A 1500 ppm stock solution of brimonidine tartrate was prepared dissolving 1.5 g of brimonidine tartrate in 1000 mL of ultrapure water. The calibration curve was constructed measuring concentrations from 2 to 15 ppm using a UV-Vis spectrophotometer (double-beam spectrophotometer with a photomultiplier tube detector JASCO V-650 UV-VIS) at wavelengths of 1 = 247 nm and 2 = 320 nm. High performance liquid chromatography (HPLC) was used to certify the precision of the UV-Vis method in brimonidine quantification. 22 Both methods gave an accuracy above 97%, thus confirming the validity of the UV-vis technique for brimonidine determination.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!