Anti bak 3814

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-BAK #3814 is a rabbit monoclonal antibody that recognizes the BAK protein. BAK is a pro-apoptotic member of the BCL-2 family of proteins and plays a key role in the intrinsic apoptosis pathway.

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Lab products found in correlation

Anti pgc1α ab54481, by Abcam (1 mentions) Anti bax 2772, by Cell Signaling Technology (1 mentions) Anti gapdh 14c10 2118, by Cell Signaling Technology (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Phosstop phosphatase inhibitor cocktail, by Roche (1 mentions) Z vad fmk, by R&D Systems (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Streptavidin hrp, by GE Healthcare (1 mentions) Anti rabbit igg haf008, by R&D Systems (1 mentions) Anti gapdh gtx100118, by GeneTex (1 mentions)

2 protocols using anti bak 3814

Protein Expression Analysis by Western Blot

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Cells were harvested by trypsination, washed in ice cold PBS and lysed in 50 mmol/l Tris-HCl, 250 mmol/l NaCl, 0.1% Triton X-100, 5 mmol/l EDTA, pH 7.6, supplemented with cOmplete protease inhibitor cocktail (Roche, Grenzach-Wyhlen, Germany) and PhosSTOP phosphatase inhibitor cocktail (Roche) followed by sonication (50 cycles, 20 sec). Equal amounts of protein were diluted in 5xLaemmli buffer, heated (95 °C, 5 min.) and separated by SDS polyacrylamide gel electrophoresis. Proteins were transferred onto 0.45 µm nitrocellulose membrane. Incubation steps were: 1 h in blocking buffer (5% non-fat dry milk; 50 mmol/l Tris-HCl; 125 mM NaCl; pH 7.0) with shaking. Washing steps were 4 × 5 min in TBST; incubation with primary antibodies was over night at 4 °C, incubation with secondary antibodies was 1 h with shaking. Primary antibodies were as follows: anti-BAX #2772, anti-BAK #3814, anti-TFAM #8076, anti-UCP2 #89326, and anti-GAPDH (14C10) #2118 (Cell Signaling, Denvers, MA, USA); anti-PGC1α #ab54481 (Abcam, Cambridge, UK).

Kleih M., Böpple K., Dong M., Gaißler A., Heine S., Olayioye M.A., Aulitzky W.E, & Essmann F. (2019). Direct impact of cisplatin on mitochondria induces ROS production that dictates cell fate of ovarian cancer cells. Cell Death & Disease, 10(11), 851.

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Antibody Sources for Western Blot Analysis

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For Western blot analysis, anti-cleaved PARP (9542), anti-cleaved Caspase 8 (9496), anti-cleaved Caspase 3 (9664), anti-Bax (2772), and anti-Bak (3814), antibodies were purchased from Cell Signaling Technology; anti-Bax (610982) antibodies were from BD Bioscience; anti-PHB1 (10787-1-AP) and anti-PHB2 (12295-1-AP) antibodies were from ProteinTech; anti-PHB1 (sc-377037), anti-PHB2 (sc-133094), anti-TOM20 (F-10) (sc-17764) monoclonal antibodies and anti-eEF2 (H-118; sc-118), anti-Drp1 (H-300; sc-32898) antibodies were from Santa Cruz Biotechnology; anti-Bak (Ab-2; TC-102) monoclonal antibody was from CALBIOCHEM; anti-ROCK1 (GTX113266) and anti-GAPDH (GTX100118) antibodies were from GeneTex; anti-FLAG antibody (018-22381) was from WAKO Pure Chemical; anti-Myc tag antibody (M192-3) was from MBL; HRP-conjugated anti-mouse IgG (HAF007), anti-rabbit IgG (HAF008) antibodies, and Z-VAD-FMK were purchased from R&D Systems; anti-GST HRP (RPN1236) and Streptavidin-HRP were obtained from GE Healthcare Life Sciences. Y27362 (ROCK inhibitor) was from Cayman Chemical. 6-Biotin-17-NAD+ (biotinyl-NAD⁺) was obtained from Trevigen. CHX was obtained from Sigma Aldrich. Pseudomonas aeruginosa exotoxin A (PEA) was from LIST Biological Laboratories, Inc.

Yahiro K., Ogura K., Terasaki Y., Satoh M., Miyagi S., Terasaki M., Yamasaki E, & Moss J. (2019). Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes. Cellular microbiology, 21(8), e13033.

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