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Rotor gene q software 1

Manufactured by Qiagen
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The Rotor-Gene Q Software 1.7 is a software package designed for use with the Rotor-Gene Q real-time PCR cycler. The software provides the necessary tools and interface to control the Rotor-Gene Q instrument and analyze the data generated from real-time PCR experiments.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Ecodry premix kit, by Takara Bio (2 mentions) Taqman fluorescent probes, by Thermo Fisher Scientific (2 mentions) Qpcr mastermix, by Eurogentec (2 mentions) Rotor gene q cycler, by Qiagen (2 mentions)

Spelling variants of this product

Rotor-Gene Q (1351 citations) Rotor-Gene (167 citations) Rotor-Gene Q software (51 citations) Rotor-Gene software (19 citations) Rotor-Gene 1.7 software (12 citations) Rotor-Gene Q Series Software 1.7 (3 citations)

2 protocols using rotor gene q software 1

1

Quantitative Analysis of Inflammatory Genes in Tissues

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Total RNAs were extracted from individual frozen tissue samples (Livers) or thioglycollate elicited peritoneal macrophages (TGEM) using RNeasy mini kit (Qiagen) as per the manufacturer’s instructions. Tissues were homogenized in RNeasy lysis buffer with a motorized homogenizer. Genomic DNA was removed by DNase I, and RNA concentration and quality were assessed with by NanoDrop. Next, 1 μg of RNA was reversely transcribed to cDNA using EcoDry Premix kit (Clontech). Real-time qPCR was carried out to determine gene expression of inflammatory molecules. All reactions were performed in the Rotor-Gene Q cycler (Qiagen) in triplicates using 50 ng of cDNA and qPCR Master Mix (Eurogentec, San Diego, CA), primers and Taqman fluorescent probes (Applied Biosystems) in a total reaction volume of 20 μL. Relative quantities of mRNA were calculated using ΔΔCt formula and two standard curves relative quantitation using Rotor-Gene Q Software 1.7 (Qiagen) with GAPDH as the reference gene.
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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2

Quantitative Analysis of Inflammatory Genes in Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNAs were extracted from individual frozen tissue samples (Livers) or thioglycollate elicited peritoneal macrophages (TGEM) using RNeasy mini kit (Qiagen) as per the manufacturer’s instructions. Tissues were homogenized in RNeasy lysis buffer with a motorized homogenizer. Genomic DNA was removed by DNase I, and RNA concentration and quality were assessed with by NanoDrop. Next, 1 μg of RNA was reversely transcribed to cDNA using EcoDry Premix kit (Clontech). Real-time qPCR was carried out to determine gene expression of inflammatory molecules. All reactions were performed in the Rotor-Gene Q cycler (Qiagen) in triplicates using 50 ng of cDNA and qPCR Master Mix (Eurogentec, San Diego, CA), primers and Taqman fluorescent probes (Applied Biosystems) in a total reaction volume of 20 μL. Relative quantities of mRNA were calculated using ΔΔCt formula and two standard curves relative quantitation using Rotor-Gene Q Software 1.7 (Qiagen) with GAPDH as the reference gene.
Que X., Hung M.Y., Yeang C., Gonen A., Prohaska T.A., Sun X., Diehl C., Määttä A., Gaddis D.E., Bowden K., Pattison J., MacDonald J.G., Ylä-Herttuala S., Mellon P.L., Hedrick C.C., Ley K., Miller Y.I., Glass C.K., Peterson K.L., Binder C.J., Tsimikas S, & Witztum J.L. (2018). Oxidized Phospholipids are Proinflammatory and Proatherogenic in Hypercholesterolemic Mice. Nature, 558(7709), 301-306.
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