All the collected blood units were screened for TTIs in accordance with national donor screening guidelines. For serological testing, the automated chemiluminescent microparticle immunoassay analyzer ARCHITECT i2000 system (CMIA, Abbott Diagnostic, USA), with validated commercially available assays, was used for the detection of HBsAg (ARCHITECT HBsAg Qualitative), Anti-HCV (ARCHITECT Anti-HCV), HIV p24 antigen and anti-HIV-1&2 (ARCHITECT HIV Ag/Ab Combo), anti-HBc (ARCHITECT Anti-HBc II), and Anti-HBs (ARCHITECT Anti-HBs). All procedures were carried out according to the manufacturer's instruction, and the results were expressed as a signal to cut-off (S/CO), and S/CO > 1.0 was considered reactive. In addition to CMIA, blood units were also tested in parallel by individual nucleic acid test (ID-NAT) for HBV-DNA, HCV-RNA and HIV-1&2-RNA using The Procleix Ultrio Elite assay on the Panther system (Grifols, Spain) as indicated by the manufacturer's instructions. For initially reactive samples, the Ultrio Elite discriminatory assays for HIV-1/2, HCV, or HBV were performed.
Anti hcv
Manufactured by Abbott
Sourced in United States
Anti-HCV is a laboratory diagnostic test used to detect the presence of antibodies to the hepatitis C virus (HCV) in human serum or plasma samples. The test is designed to identify individuals who have been exposed to the HCV virus, providing essential information for the diagnosis and management of hepatitis C infection.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using anti hcv
1
Blood Donor Screening for Transfusion-Transmissible Infections
2
HCV RNA Detection from DBS using Water Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCV-RNA from DBS was extracted with either lysis buffer, protease K or plain, purified H2O. Plain water turned out to give the best yield with our RNA extraction procedure. One DBS was dissolved in 1mL H2O overnight. The samples were spun down at 10.800 G for 10 minutes.
We tested 500 and 100 μL of the supernatant, and 100 μL gave the best yield, whereas 500 μL resulted in some inhibition. Total RNA was isolated from 100 μL supernatant using NucliSENS easyMAG Magnetic Silica (bioMérieux, Marcy l’Etoile, France). Detection and quantification of viral nucleotide sequences were performed by In house real-time PCR using molecular beacons on a Mx3005P Real-Time PCR System (Stratagene, La Jolla, USA). We also tested our standard routine method Abbott RealTime HCV, but were not able to get meaningful results. Antibodies against HCV (anti-HCV) were tested using our routine chemiluminescence assay (anti-HCV, Architect; Abbott Laboratories, Abbott Park, IL, USA) according to the manufacturer’s recommendation only using supernatant instead of plasma.
We tested 500 and 100 μL of the supernatant, and 100 μL gave the best yield, whereas 500 μL resulted in some inhibition. Total RNA was isolated from 100 μL supernatant using NucliSENS easyMAG Magnetic Silica (bioMérieux, Marcy l’Etoile, France). Detection and quantification of viral nucleotide sequences were performed by In house real-time PCR using molecular beacons on a Mx3005P Real-Time PCR System (Stratagene, La Jolla, USA). We also tested our standard routine method Abbott RealTime HCV, but were not able to get meaningful results. Antibodies against HCV (anti-HCV) were tested using our routine chemiluminescence assay (anti-HCV, Architect; Abbott Laboratories, Abbott Park, IL, USA) according to the manufacturer’s recommendation only using supernatant instead of plasma.
3
Baseline Biochemical Profiling in Clinical Trials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Baseline biochemical values including alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (CRE), fasting blood glucose (GLU-AC), platelet count, total cholesterol (T-CHO), high-density lipoprotein (HDL), low- density lipoprotein (LDL), and triglyceride (TG) were collected at enrollment. Laboratory assays were performed using commercially standardized automated techniques. The serum levels of HBeAg, anti-HBe, anti-HBs, anti-HCV, and anti-HDV were tested using commercial kits (Abbott Laboratories, Abbott Park, IL, USA).
4
Criteria for Hepatocellular Carcinoma Diagnosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The diagnosis of HCC was made based on the findings of radiological characteristics in at least 2 imaging methods including ultrasound, liver angiography, magnetic resonance imaging, and contrast-enhanced four-phase dynamic computed tomography. Alternatively, the diagnosis was confirmed by 1 positive imaging modality along with serum α-fetoprotein (AFP) level > 400 ng/mL or biopsy confirmed as previously described.1 (link),8 (link),10 ,11 (link) Patients were considered to have hepatitis C virus (HCV) infection if they were seropositive for antibody against HCV (anti-HCV, Abbott Laboratories). Hepatitis B virus (HBV) infection was diagnosed if patients were seropositive for hepatitis B surface antigen (HBsAg) (Abbott Laboratories). Alcoholism was diagnosed in patients with daily consumption of at least 40 g of alcohol for 5 years or more.10 ,11 (link) PS was determined at enrollment defined by to the Eastern Cooperative Oncology Group.12 (link) The modification of diet in renal disease formula was used to calculate estimated glomerular filtration rate (eGFR). We estimated total tumor volume by using mathematical formulas as indicated in our previous studies.13 (link)
5
Comprehensive Infectious Disease Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All participants had a sample taken to test for HIV, HBV, HCV and syphilis . HIV 1 and 2 antibodies and p24 antigen were tested using Abbott Architect Ag/Ab Combo. Hepatitis B core antibody (HBcAb) was tested using Abbott Architect Anti-HBc II. If this was positive, the sample was tested for Hepatitis B surface antigen (HBsAg). Hepatitis C Antibodies were tested using Abbott Architect Anti-HCV. Treponema pallidum antibody (Anti-TP) was tested using Abbott Architect Syphilis TP; if this was positive, Venereal Disease Research Laboratory VDRL was done. Participants were considered to have syphilis requiring treatment if both tests were positive.
Each of the initial tests above was completed using Chemiluminescent Immunoassay (CLIA) in pools of 4 participant samples. If a pool was positive, each sample was tested individually. This reduced the cost without reducing the sensitivity, as reported previously [18 ,19 ].
In addition, those identified as at risk of diabetes or hypercholesterolemia had samples taken for random blood glucose and lipid profile.
Each of the initial tests above was completed using Chemiluminescent Immunoassay (CLIA) in pools of 4 participant samples. If a pool was positive, each sample was tested individually. This reduced the cost without reducing the sensitivity, as reported previously [18 ,19 ].
In addition, those identified as at risk of diabetes or hypercholesterolemia had samples taken for random blood glucose and lipid profile.
6
Hepatocellular Carcinoma and Chronic Liver Disease Serology
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!