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3 μm chromxp c18cl

Manufactured by AB Sciex
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The 3 μm ChromXP C18CL is a high-performance liquid chromatography (HPLC) column. It features 3 μm silica-based particles coated with a C18 stationary phase. This column is designed for the separation and analysis of a wide range of organic compounds.

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Lab products found in correlation

425 system, by AB Sciex (2 mentions) 5 μm chromxp c18cl, by AB Sciex (2 mentions) Lcms grade solvents, by Honeywell (2 mentions) Tripletof 5600 mass spectrometer, by AB Sciex (2 mentions)

Spelling variants of this product

ChromXP (11 citations)

2 protocols using 3 μm chromxp c18cl

1

Peptide Separation and Analysis by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Eksigent 425 system was coupled to an AB Sciex TripleTOF 5600+ mass spectrometer. The liquid chromatography system uses a trap column (ChromXP 5 μm ChromXP C18CL 120 Å 10×0.3mm) and an analytical column (Eksigent 3 μm ChromXP C18CL 120 Å 150×0.3mm). The analytical column temperature was maintained at 35 °C. LCMS grade solvents were purchased from Honeywell and composed of buffer A (0.1% formic acid in water (v/v)) and buffer B (0.1% formic acid in acetonitrile (v/v)). Peptides were reconstituted in 10μl 2% solvent B and 8μl was loaded at 10 μl/min for 3 minutes onto the trap column. Analytical separation was established by maintaining 3% B during loading. After a valve switch event, peptides were resolved using the analytical column at a flow rate of 5 μl/min with a linear gradient of 3–25% HPLC buffer B for 38 minutes, followed by a 5 min linear gradient to 32% B. Following the peptide elution window, in 2 min the gradient was increased to 80% B for 3 min. Initial chromatographic conditions were restored in 1 min and maintained for 8 min.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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2

Peptide Separation and Analysis by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols
An Eksigent 425 system was coupled to an AB Sciex TripleTOF 5600+ mass spectrometer. The liquid chromatography system uses a trap column (ChromXP 5 μm ChromXP C18CL 120 Å 10×0.3mm) and an analytical column (Eksigent 3 μm ChromXP C18CL 120 Å 150×0.3mm). The analytical column temperature was maintained at 35 °C. LCMS grade solvents were purchased from Honeywell and composed of buffer A (0.1% formic acid in water (v/v)) and buffer B (0.1% formic acid in acetonitrile (v/v)). Peptides were reconstituted in 10μl 2% solvent B and 8μl was loaded at 10 μl/min for 3 minutes onto the trap column. Analytical separation was established by maintaining 3% B during loading. After a valve switch event, peptides were resolved using the analytical column at a flow rate of 5 μl/min with a linear gradient of 3–25% HPLC buffer B for 38 minutes, followed by a 5 min linear gradient to 32% B. Following the peptide elution window, in 2 min the gradient was increased to 80% B for 3 min. Initial chromatographic conditions were restored in 1 min and maintained for 8 min.
Edwinson A.L., Yang L., Peters S., Hanning N., Jeraldo P., Jagtap P., Simpson J.B., Yang T.Y., Kumar P., Mehta S., Nair A., Breen-Lyles M., Chikkamenahalli L., Graham R.P., De Winter B., Patel R., Dasari S., Kashyap P., Griffin T., Chen J., Farrugia G., Redinbo M.R, & Grover M. (2022). Gut Microbial β-Glucuronidases Regulate Host Luminal Proteases and are depleted in Irritable Bowel Syndrome. Nature microbiology, 7(5), 680-694.
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