Cd64 clone x54 5 7

Manufactured by BioLegend

Sourced in United States

CD64 (clone X54-5/7.1) is a lab equipment product offered by BioLegend. It is a monoclonal antibody that binds to the CD64 antigen. CD64 is a high-affinity Fc receptor for IgG.

Automatically generated - may contain errors

Lab products found in correlation

Cd11b clone m1 70, by BioLegend (6 mentions) Ly6c clone hk1, by BioLegend (5 mentions) Cd45 clone 30 f11, by BioLegend (5 mentions) Mhcii clone m5 114, by BioLegend (3 mentions) Cd11c clone n418, by BioLegend (3 mentions) Facscanto, by BD (2 mentions) Cx3cr1 clone sa011f11, by BioLegend (2 mentions) Cd45.2 clone 104, by BioLegend (2 mentions) Ly6g 1a8, by BioLegend (2 mentions) Cd103 clone 2e7, by BioLegend (2 mentions) Zombie violet fixable dye, by BioLegend (2 mentions) Cd45.1 clone a20, by BioLegend (2 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Diva software, by BD (1 mentions) Mertk, by R&D Systems (1 mentions) Cd11c n418, by BioLegend (1 mentions) Cd206 clone c068c2, by BioLegend (1 mentions) Fixable viability dye, by Thermo Fisher Scientific (1 mentions) Anti ly6g clone 1a8, by BioLegend (1 mentions) Anti f4 80 clone bm8, by BioLegend (1 mentions)

Close spelling variants of this product

CD64 (X54-5/7.1, (16 citations) X54-5/7.1 (8 citations) Clone X54–5/7.1 (3 citations) CD64 APC clone X54–5/7.1 (3 citations) Anti-CD64 (clone X54-5/7.1; (2 citations)

8 protocols using cd64 clone x54 5 7

Macrophage Phenotyping and DsRed Degradation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For macrophage analysis, CD45.1 (Ly-5.1) and CD45.2 (Ly-5.2; Tonbo Bioscience), CD11b (clone M170; BioLegend), F4/80 (clone CIA31; Serotec), Mertk (R&D Systems), CD64 (clone X54-5/7.1; BioLegend), CD11c (N418; BioLegend), and MHCII (clone M5/114.15.2; BioLegend) antibodies were used. For validation experiments, CD206 (clone C068C2; BioLegend), CD163 (clone M96; Santa Cruz Biotechnology, Inc.), and Tim4 (clone RMT4-54; BioLegend) antibodies were used. Cells were stained with the indicated antibodies for 15 min at 4°C and were analyzed by fluorescence activated cell sorting (FACS) on an LSR Fortessa flow cytometer equipped with DIVA software (BD). FlowJo software was used to analyze data. To measure the degradation of the DsRed fluorescence signal within macrophages, extracted tissues were processed (as explained above) and kept at 37°C or 4°C (control) during flow cytometric analysis of macrophages every hour for a total of 6 h.

A-Gonzalez N., Quintana J.A., García-Silva S., Mazariegos M., González de la Aleja A., Nicolás-Ávila J.A., Walter W., Adrover J.M., Crainiciuc G., Kuchroo V.K., Rothlin C.V., Peinado H., Castrillo A., Ricote M, & Hidalgo A. (2017). Phagocytosis imprints heterogeneity in tissue-resident macrophages. The Journal of Experimental Medicine, 214(5), 1281-1296.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry for Lung Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

BAL cells and lung single cell suspensions were first incubated with anti-mouse FcγIII/II receptor mAb (clone 2.4G2) for 15 min. The Fc receptor-blocked cells were further stained with Fixable Viability Dye (eBioscience, Waltham, MA) to label dead cells. Cells were stained for surface markers using the following mAbs: anti-CD11b (clone M1/70; eBioscience), anti-Ly6G (clone 1A8; Biolegend, San Diego, CA), anti-CD11c (clone HLC; BD Biosciences, San Jose, CA), anti-F4/80 (clone BM8; Biolegend), anti-Siglec F (clone E50-2440; BD Bioscience), MHC Class II (clone M5/114.15.2; Biolegend), CD24 (clone M1/69; Biolegend), CD64 (clone X54-57.1; Biolegend), Ly6C (clone HK1.4; Biolegend). Stained cells were analyzed on a FACSCanto (BD Biosciences) and the data were analyzed using FlowJo software (Tree Star Inc. Ashland, OR).

Califano D., Furuya Y, & Metzger D.W. (2018). Effects of influenza on alveolar macrophage viability are dependent on mouse genetic strain. Journal of immunology (Baltimore, Md. : 1950), 201(1), 134-144.

+ Open protocol

+ Expand

Sorting and Sequencing Microglia from Embryonic Tissues

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following single-cell suspension preparation, cells were pelleted and subsequently stained with fluorophore-conjugated antibodies against CD11b (clone M1/70), CD45 (clone 30-F11), CD45.1 (clone A20), CD45.2 (clone 104), CX3CR1 (clone SA011F11; BioLegend), F4/80 (clone BM8; BioLegend), Ly6C (clone HK1.4), Ly6G (clone 1A8; BioLegend), Gr-1 (clone RB6-8C5), CD115 (clone AFS98), MHC II (clone M5/114.15.2), CD11c (clone N418), CD64 (clone X54-5/7.1; BioLegend), CD86 (clone GL1; BioLegend), Tmem119 (clone 106–6; Abcam), and either DAPI or propium iodide viability dyes (all from eBioscience if not indicated otherwise). Flow cytometry was performed using a Fortessa analyzer (BD Biosciences), and FACS was performed using a FACS Aria II (BD Biosciences) or LSRII (BD Biosciences). The gating strategies used for flow cytometry analysis of embryonic tissues are adapted from Hoeffel et al. (2015) (link) and shown in Fig. S5. Resident microglia were sorted as doublet⁻DAPI⁻CD11b⁺CD45^int (Fig. S2 B). Flow cytometry data analysis was performed using FlowJo (TreeStar) software. For ULI RNA-seq, microglia were double-sorted to reach a purity of >98%, and 1,000 cells were sequenced.

Kana V., Desland F.A., Casanova-Acebes M., Ayata P., Badimon A., Nabel E., Yamamuro K., Sneeboer M., Tan I.L., Flanigan M.E., Rose S.A., Chang C., Leader A., Le Bourhis H., Sweet E.S., Tung N., Wroblewska A., Lavin Y., See P., Baccarini A., Ginhoux F., Chitu V., Stanley E.R., Russo S.J., Yue Z., Brown B.D., Joyner A.L., De Witte L.D., Morishita H., Schaefer A, & Merad M. (2019). CSF-1 controls cerebellar microglia and is required for motor function and social interaction. The Journal of Experimental Medicine, 216(10), 2265-2281.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated cells were treated with 2% FBS/PBS containing Fc block (BD Biosciences) for 20 min at 4°C and then stained with fluorescent-conjugated antibody. Antibodies used for flow cytometry analysis are as follows: CD11c (clone N418; eBiosciences), CD64 (clone X54-5/7.1; Biolegend), CD103 (clone M290; BD Biosciences), F4/80 (clone BM8; eBiosciences), CD11b (clone M1/70; BD Biosciences), CX₃CR1 (clone SA011F11; Biolegend), CD4 (clone RM4-5; eBiosciences), CD8α (clone 53–6.7; BD Biosciences), OVA-AF647 (Molecular Probe). Data were obtained using FACSCanto (BD Biosciences) and analyzed with FlowJo software (Tree Star, Ashland, OR).

Kim Y.I., Song J.H., Ko H.J., Kweon M.N., Kang C.Y., Reinecker H.C, & Chang S.Y. (2018). CX3CR1+ macrophages and CD8+ T cells control intestinal IgA production. Journal of immunology (Baltimore, Md. : 1950), 201(4), 1287-1294.

+ Open protocol

+ Expand

Flow Cytometry Antibody Panel for Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Distributors and origin of reagents, equipment, and materials used in this study are indicated in the Methods section.
Reagents and antibodies used for flow cytometry and cell sorting: Zombie Violet fixable dye (BioLegend), CD45 (clone 30-F11, BioLegend), CD45.1 (clone A20, BioLegend), CD45.2 (clone 104, BioLegend), CD103 (clone 2E7, BioLegend), Ly-6G (1A8, BioLegend), CD64 (clone X54-5/7.1, BioLegend), CD11b (clone M1/70 BioLegend), CD11c (clone N418, BioLegend) Ly-6C (clone HK1.4, BioLegend), MHC II (clone M5/114.15.2, BioLegend).

Patik I., Redhu N.S., Eran A., Bao B., Nandy A., Tang Y., El Sayed S., Shen Z., Glickman J., Fox J.G., Snapper S.B, & Horwitz B.H. (2023). The IL-10 receptor inhibits cell extrinsic signals necessary for STAT1-dependent macrophage accumulation during colitis. Mucosal immunology, 16(3), 233-249.

+ Open protocol

+ Expand

Flow Cytometry Antibody Panel for Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lyons R., Payne C., McCabe M, & Fielder C. (1998). Legibility of doctors’ handwriting: quantitative comparative study. BMJ : British Medical Journal, 317(7162), 863-864.

+ Open protocol

+ Expand

Multicolor Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibiotics vancomycin, polymyxin B, ampicillin, and neomycin were obtained from Gold Biotechnology, Inc. (St. Louis, MO), and metronidazole was from Sigma-Aldrich Corp. (St. Louis, MO). Liposomal clodronate and control liposomes were obtained from Encapsula NanoSciences LLC (Brentwood, TN). The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet^TM fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11, BioLegend), CD11b (clone M1/70, BioLegend), CD11c (clone N418, BioLegend), CD103 (clone 2E7, eBioscience/ Thermo Fisher Scientific, Waltham, MA), CD64 (clone X54-5/7.1, BioLegend), I-A/I-E (MHCII, clone M5/114.15.2, BioLegend), Ly6C (clone HK1.4, BioLegend), CD3e (clone 145–2 C11, BioLegend), CD4 (clone GK1.5, BioLegend), FoxP3 (clone FJK-16s, eBioscience), IFNγ (clone XMG1.2, BioLegend), IL17A (clone TC11-18H10.1, BioLegend), CD127 (clone A7R34, BioLegend), CD90.2 (Thy1.2, clone 30-H12, BioLegend), and RORγt (clone B2D, eBioscience).

Redhu N.S., Bakthavatchalu V., Conaway E.A., Shouval D.S., Tsou A., Goettel J.A., Biswas A., Wang C., Field M., Muller W., Bleich A., Li N., Gerber G.K., Bry L., Fox J.G., Snapper S.B, & Horwitz B.H. (2017). Macrophage dysfunction initiates colitis during weaning of infant mice lacking the interleukin-10 receptor. eLife, 6, e27652.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following anti-mouse antibodies were used (dilutions are indicated): CD45 (clone 30-F11, 1:100), CD11b (clone M1/70, 1:300), Ly6C (clone HK1.4, 1:300), CD115 (clone AFS98, 1:100), CD64 (clone X54-5/7.1, 1:50), Ly6G (clone 1A8, 1:100), and Tim4 (clone RMT4-54, 1:50), MHCII (clone M4-114.15.2, 1:200), CX₃CR1 (clone SA011F11, 1:100), CD14 (clone M5E2, 1:100), CD16 (clone 3G8, 1:100) all purchased from BioLegend, San Diego, CA, USA. Anti-mouse F4/80 (clone A3-1, 1:50) was purchased from BIORAD. Cells were analyzed with BD FACSCanto™ II (BD Bioscience). Flow cytometry analysis was performed using FlowJo software (TreeStar, Ashland, OR, United States).

Mouhadeb O., Ben Shlomo S., Cohen K., Farkash I., Gruber S., Maharshak N., Halpern Z., Burstein E., Gluck N, & Varol C. (2018). Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function. Frontiers in Immunology, 9, 2623.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!