Tissue sections were stained with rat monoclonal anti-Ly6C (IgG2c; eBioscience, Paisley, UK) followed by Alexa Fluor 568-conjugated anti-rat IgG (Invitrogen, Paisley, UK). Then counter stained with anti-CD11b-FITC (eBioscience, Cheshire, UK) followed by goat anti-FITC-conjugated with Alexa Fluor488 (Invitrogen, Paisley, UK). Sections were washed with PBS, coverslip mounted and imaged on a Leica SP5C inverted confocal laser scanning microscope.
Alexa fluor 568 conjugated anti rat igg
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Alexa Fluor 568-conjugated anti-rat IgG is a secondary antibody used for detection in immunoassays. It is conjugated to the Alexa Fluor 568 fluorescent dye, which has an excitation maximum of 578 nm and an emission maximum of 603 nm.
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2 protocols using alexa fluor 568 conjugated anti rat igg
1
Ly6C and CD11b Immunostaining
2
Immunofluorescence Tissue Analysis
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Tissue sections were fixed in 4% paraformaldehyde, depleted of paraffin, and rehydrated using a graded ethanol series. Thereafter, the sections were subjected to antigen retrieval by heating for 10 minutes at 105°C in Target Retrieval Solution (pH 9.0; Dako, Tokyo, Japan) and incubated overnight at 4°C with a primary antibody. Immunoreactivity was detected using Alexa Fluor 568–conjugated anti-rat IgG and Alexa Fluor 488–conjugated anti-rabbit IgG (Invitrogen). Samples were examined using an LSM710 Zeiss confocal microscope.
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