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3 protocols using cericammonium nitrate can

1

Chitosan-Based Composite Synthesis

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Chitosan powder (with 75–85% degree
of deacetylation and 190 000–310 000 kg/mol molecular
weight) was obtained from Sigma-Aldrich. Also, METMS, METAC, ceric
ammonium nitrate (CAN), methanol, sodium hydroxide pellets, poly diallyldi
methylammonium, acetic acid (90 vol %), HCl (37%), sulfuric acid (98
wt %), and direct yellow 50 (Y50) dye were purchased from Sigma-Aldrich
company. Potassium polyvinyl sulfate (PVSK) (with molecular weight
of 200–300 kg/mol, 98.4% esterified) was provided by Wako Pure
Chemical Industries Ltd., Japan. Cellulose acetate dialysis membrane
(molecular weight cut-off of 1000 g/mol) was purchased from Wako Chemicals,
Japan. In this research work, no unexpected or unusually high safety
hazards were encountered.
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2

Polymer Functionalization with Maleic Anhydride and IEM

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The different types of chemicals and modifiers used in this study are as follows: maleic anhydride (MAH, 95%, Sigma-Aldrich, St. Louis, MO, USA) and 2-Isocyanatoethyl methacrylate (IEM, 98%, Sigma-Aldrich, St. Louis, MO, USA) are used as functional groups; ceric ammonium nitrate (CAN, Sigma-Aldrich, St. Louis, MO, USA); dicumyl peroxide (DCP, 98%, Fisher, Portsmouth, NH, USA) is used as an initiator, acetone (99%, Sigma-Aldrich, St. Louis, MO, USA) is used as a solvent. All the chemicals were used as received without any modification, and all the functionalisation experiments were performed in a heterogenous mixture (polymer and/or filler and solvent) in a round bottom flask with a magnetic stirrer equipped with a condenser.
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3

Chitosan-based Hydrogel for Controlled Drug Delivery

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Chitosan 99% DDA (Sigma Aldrich), nitric acid (65%, d=1.40 g/mL, Merck), acrylamide (AAm) (Merck), methylenebisacrylamide (MBA) (Fluka), sodium hydroxide (Merck), acetylsalicylic acid (ASA) (Sigma-Aldrich), glacial acetic acid (Merck), isopropyl alcohol (technical grade, distilled prior to every use), acetone (Merck). Rest of the chemicals were all of Merck brand and used as received.
Ceric ammonium nitrate (CAN) was Sigma-Aldrich brand and it was used as a stock solution of 0.05 M in 0.1 M nitric acid.
Acidic buffer solution (pH 2) was prepared dissolving KCl (7.45 g) in water; then HCl (37%, 1.75 mL) was added to the mixture and completed to 1.0 L with distilled water.
Slightly acidic buffer solution (pH 6) was prepared dissolving Na2HPO4•7 H2O (1.307 g) and NaH2PO4•H2O (13.127 g) in water; then the mixture completed up to 1.0 L with distilled water.
Basic buffer solution (pH 8.5) was prepared dissolving H3BO3 (61.83 g) and NaOH (10.00 g) in water; then the mixture completed to 1.0 L with distilled water.
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