Gv358 pcdh9 lentivirus

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and phosphate buffered saline (PBS) (pH = 7.2) were purchased from Gibco (Shanghai, China); ethanol (70%), isopropyl alcohol, and Triton X-100 were bought from Sigma-Aldrich (Shanghai, China); Cell Counting Kit-8 (CCK-8) were bought from Dongren Chemical Technology (Shanghai, China); bicinchonininc acid (BCA) protein assay kit was purchased from Thermo Fisher Scientific (Shanghai, China); antibodies were purchased from Cell Signaling Technology (Shanghai, China) and Abcam (Shanghai, China); the primers [circ_0084043, PCDH9, RAC1, Cyclin D1, Pyk2, FAK, MMP2, MMP9, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)] were designed and synthesized by Sangon Biotech (Shanghai, China); the small interfering ribonucleic acid (siRNA) for circ_0084043 (si-circ_0084043) was designed and synthesized by Ribo Biotech (Guangzhou, China); the miR-134-5p mimics, miR-134-5p inhibitor, and miRNA negative control (miR-NC) were also purchased from Ribo Biotech; GV358-PCDH9 lentivirus and GV358-siRNA lentivirus were designed by Genechem (Shanghai, China); SYBR^® Premix Ex Taq™ II and PrimeScript™ RT reagent Kit with genomic DNA (gDNA) Eraser were bought from Takara Bio Inc. (Beijing, China); ultrapure water was obtained from EPED-20TF (Nanjing, China).

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), phosphate buffer solution (PBS) (pH = 7.2), Diethyl Pyrocarbonate (DEPC)-treated water (Ambion), and TRIzol reagent (Invitrogen) were purchased from Gibco (Thermo Fisher Scientific, Shanghai, China); ethanol (70%), isopropyl alcohol, and Triton X-100 were bought from Sigma-Aldrich (Shanghai, China); Cell Counting Kit-8 (CCK-8) was bought from Dongren Chemical Technology (Shanghai, China); GV358-PCDH9 lentivirus and GV358-siRNA (short interfering RNA) lentivirus were designed by GeneChem (Shanghai, China); SYBR^® Premix Ex Taq™ Ex Taq ™ II and PrimeScript™ RT reagent Kit with gDNA Eraser were bought from Takara Bio Inc. (Beijing, China); water was obtained from EPED-20TF (Nanjing, China).