Endogenous detection of Fbp-Camk2α interaction was performed using the Duolink® In Situ Orange Starter Kit Mouse/Rabbit (Merck KGaA, Darmstadt, Germany) according to the protocol provided by the manufacturer, using mouse anti-Fbp (1:500, Santa Cruz Biotechnology, Dallas, TX, USA, sc-271799) and rabbit anti-Camk2α (1:500, Merck KGaA, Darmstadt, Germany, C6974) primary antibodies. In controls, the primary antibodies were omitted.
Rabbit anti camk2α
Manufactured by Merck Group
Sourced in United States
Rabbit anti-Camk2α is a primary antibody product that specifically targets the Calcium/calmodulin-dependent protein kinase II alpha (CaMK2α) protein. CaMK2α is a serine/threonine-protein kinase that is involved in the regulation of various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to detect and study the CaMK2α protein.
Automatically generated - may contain errors
Lab products found in correlation
Duolink in situ orange starter kit mouse rabbit, by Merck Group (2 mentions)
Mouse anti fbp, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse peroxidase conjugated antibodies, by Merck Group (1 mentions)
Mouse anti β actin, by Merck Group (1 mentions)
Mouse anti fbp2, by Santa Cruz Biotechnology (1 mentions)
3 protocols using rabbit anti camk2α
1
Endogenous Fbp-Camk2α Interaction Detection
2
Detecting Neuronal Protein Expression
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Western blot of neuronal protein extracts was performed as described previously [48 (link)]. The following primary antibodies were used: rabbit anti-Fbp (1:500, isolated and purified as described in [49 (link)]), rabbit anti-Camk2α (1:1000, Merck KGaA, Darmstadt, Germany, C6974) and mouse anti-β-actin (1:50,000, Merck KGaA, Darmstadt, Germany, A1978). The following secondary antibodies were used: goat anti-rabbit peroxidase-conjugated (1:50,000, Merck KGaA, Darmstadt, Germany, a0545) and goat anti-mouse peroxidase-conjugated antibodies (1:100,000, Merck KGaA, Darmstadt, Germany, a9044) were used. The reaction was visualized chemiluminescently.
3
Fbp-Camk2α Interaction Visualization
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Detection and visualization of Fbp-Camk2α interaction was performed using the DuoLink® In Situ Orange Starter Kit Mouse/Rabbit (Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instruction, using mouse anti-Fbp2 (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA, sc-271799) and rabbit anti-Camk2α (1:1000, Merck KGaA, Darmstadt, Germany, C6974) primary antibodies. In controls, the primary antibodies were omitted.
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