Trima accel

Plateletpheresis procedures were performed on two types of cell separators (TRIMA ACCEL^®, Terumo BCT Lakewood, Colorado and AMICUS^®, Fresenius Kabi, Germany). The mean platelet yield of collected platelet product was ≥3 × 10¹¹ platelets, fulfilling the requisite quality control criteria.[2 ]
Before the procedure, the donor was briefed about the procedure and a written informed consent was obtained. Antecubital veins on both sides were assessed for their suitability for phlebotomy. Sampling was done from the dorsum of the hand for blood grouping and transfusion transmissible infections screening of the donor. For the evaluation of serum TPO levels and platelet counts, 10-ml blood was drawn (2 ml in EDTA vacutainer and an 8 ml of blood in plain vacutainer) before platelet donation, at the end of the donation (apheresis), and at the 3^rd- and 5^th- postdonation day. At each time point, platelet parameters, including platelet count, mean platelet volume (MPV), and platelet distribution width (PDW), were estimated using ORION 60™ hematology analyzer.
Serum TPO levels were determined using quantitative sandwich enzyme-linked immunosorbent assay technique (Raybiotech, USA) on stored serum samples, as per the protocol of the manufacturer.

Apheresis platelet concentrates of blood group O stored for a median of 1 day (range: 1‐3) were used. The platelet concentrates were produced by the blood bank at Sahlgrenska University Hospital using an apheresis device (Trima Accel; Terumo BCT Europe, Zaventem, Belgium) in accordance with European guidelines.17 Autologous plasma was used as storage medium. The mean platelet count in the platelet concentrates was 1747 ± 41 × 10⁹ L⁻¹. In vitro addition of 120 × 10⁶ platelets to a 1‐mL blood sample was tested. This corresponds to an increase in platelet count achieved by transfusion of approximately three apheresis units to a 70‐kg patient. By using the platelet concentration in the individual concentrate, the specific volume to add from the concentrate was calculated. The mean volume of platelet concentrate added was 69 ± 1.6 μL.

The blood center had the MCS+ (software version 2-UPP-A.2-IE, Haemonetics, Braintree MA, USA), Trima Accel, (software version 6.0, Terumo BCT, Lakewood, CO, USA) and Spectra Optia (Terumo BCT, Lakewood, CO, USA) equipment for platelet apheresis. The universal platelet protocol in the MCS+ equipment uses intermittent flow centrifugation in which the whole blood is pumped into the spinning bowl and platelet components are pushed upward and inward to the appropriate bag during each cycle. The system then concentrates the platelet product and suspends it in the platelet additive solution. The Trima Accel and Spectra Optia equipment works on continuous flow centrifugation and the leukoreduction system (LRS) chamber separates platelets from white blood cells using the elutriation principle. Donors underwent collection in one of these apheresis equipment. The donation endpoint, i.e., target yield, was set based on the estimated procedural time, donor comfort during the procedure, donor complications and estimated post-donation platelet count. The maximum procedure duration was set below 130 minutes for all the procedures. The blood center preferred donors with higher platelet counts for the MCS+ equipment due to the longer procedure duration.

Recovered COVID-19 patient who had received a COVID-19 diagnosis by the Québec Provincial Health Authority and met the donor selection criteria for plasma donation in use at Héma-Québec were recruited. The participant was allowed to donate plasma at least 14 days after complete resolution of COVID-19 symptoms. A volume of 500 mL–750 mL of plasma was collected by plasmapheresis (TRIMA Accel, Terumo BCT). Disease severity (date of symptoms onset, end of symptoms, type, and intensity of symptoms, need for hospitalization/ICU) was documented using a questionnaire administered at the time of recruitment. For additional details of CCP-5 (sex, age, blood group of the convalescent donor and day of collection post infection, please refer to key resources table.