ICLASS was performed as previously reported.73 (link) An illuminated open field (40 cm × 40 cm, L × W) was used for this behavioral experiment. Within the open field, red lines marked a central area (20 cm × 20 cm, L × W). Before behavioral sessions, mice were gently attached to a fiber optic patch cord with optical fiber via a ceramic sleeve (Hangzhou Newdoon Technology). Optical stimulation was controlled by a computer running Ethovision XT software (Noldus, Wageningen, Netherlands), which videotaped and analyzed location and movements in the central and the periphery arena. The ICLASS task began 3 weeks after AAV injection. The mouse was gently released from the center of the open field and allowed for exploration for 15 min, and the locomotion was monitored and calculated. Whenever the centroid of the mouse body was located within the central or the periphery arena, blue light pulses were passed to the D1NAc-VM and D1NAc-VP projections through the optical fiber (ChR2: 473 nm, 10 mW, 5 ms pulse at 20 Hz upon entry). Mouse movements and the percent ratio of the time spent in the central and the periphery arena were tracked and analyzed by Ethovision XT software (Noldus, Wageningen, Netherlands).
Ethovision xt software
Manufactured by Noldus
Sourced in Netherlands, United States, Japan, Canada, Germany
EthoVision XT is a video tracking software that automatically tracks and analyzes the movement and behavior of animals. It captures and records the position and movement of animals in real-time and generates quantitative data for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Daniovision observation chamber, by Noldus (11 mentions)
Daniovision system, by Noldus (7 mentions)
Computer linked video camera, by Imaging Source (6 mentions)
Daniovision, by Noldus (5 mentions)
Daniovision observational chamber, by Noldus (3 mentions)
Wireless low pro le running wheels, by Med Associates (2 mentions)
Daniovision tracking system, by Noldus (2 mentions)
Danioscope software, by Noldus (2 mentions)
Daniovision device, by Noldus (2 mentions)
Ultrasoundgate, by Avisoft (2 mentions)
Recorder usgh software, by Avisoft (2 mentions)
Ultrasoundgate player 116, by Avisoft (2 mentions)
Aca1300 60gc, by Basler (2 mentions)
Eight arm radial maze, by Med Associates (2 mentions)
Phenotyper, by Noldus (2 mentions)
Wv cl930, by Panasonic (2 mentions)
Daniovision video tracking system, by Noldus (2 mentions)
Sound proof setup, by Noldus (2 mentions)
R software, by R Project for Statistical Computing (2 mentions)
Topscan software, by CleverSys (2 mentions)
230 protocols using ethovision xt software
1
ICLASS Behavioral Assay with Optogenetic Stimulation
2
Open Field Test for Measuring Spontaneous Hyperactivity in Rats
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Spontaneous hyperactivity in rats in response to novel environments is useful for assessing psychomotor agitation (a symptom of SCZ and MDpsy) (Moller et al., 2015 (link), Schatzberg and Rothschild, 1992 (link)). The method of Sherif and Oreland, 1995 (Sherif and Oreland, 1995 (link)) was used to determine the total distance travelled (cm) in the open field test. Rats were placed individually into the centre of a square arena (100 ×100 x 50 cm). The test was conducted in a dimly lit room illuminated with red light (40 W). Animal behaviour was recorded for 5 min using a ceiling-mounted digital camera. The video files were analysed using Noldus Ethovision XT software (Noldus® Information Technology, Wageningen, The Netherlands).
3
Behavioral Analyses of Mice and Rats post LPS
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Mice were single housed with wireless low-profile running wheels (Med Associates, Fairfax, VT) to measure voluntary wheel running activity, which was quantified as total number of rotations per night (day running is not reported as mice display minimal activity during the day). Running wheels were provided to mice for 10–12 days prior to the initial LPS or PBS treatment to allow the mice to develop stable baseline running behavior. Locomotor activity in a new environment was measured for 5 min after mice were individually placed in an empty rectangular arena (18.4 × 29.2 cm). Activity was recorded by a video camera, and distance traveled was quantified using the Noldus Ethovision XT Software (Noldus Information Technology, Leesberg, VA).
Open-field behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-field box of 65 × 65 × 65 cm and filmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety, and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
Open-field behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-field box of 65 × 65 × 65 cm and filmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety, and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
4
Social Interaction Test Protocol
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SI was performed according to a previously described protocol (Golden et al., 2011 (link)). SI tests were conducted under red light in a testing arena (41 × 41 × 40 cm), with a removable stimulus cage (10 × 10 × 10 cm) used to hold the social stimulus animal. There were two, 2.5-min trials. During the first trial, the experimental mouse was allowed to explore the arena with an empty stimulus cage. The mouse was then removed from the arena, and a social partner (CD1, unless otherwise noted) was placed inside the stimulus cage. The experimental mouse was returned to the arena for another 2.5 min. Each session was recorded and exploration of the whole arena, as well as the exploration of the cage/interaction zone was scored using automatic tracking software (Noldus Ethovision XT software; Noldus Information Technology, Leesburg, VA). The SI Ratio was calculated as time spent in the interaction zone with CD1 present/not present. A social interaction score of ≤1 indicates susceptible, while >1 indicates resilient (Assessed in Cohorts 1–4).
5
Spatial Memory Assessment in Mice
6
Nighttime Climbing Behavior Monitoring
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Climbing activity was monitored each night between 8 PM and 6 AM using a Reconyx game camera (Reconyx, Inc., Holmen, WI). Photos were taken every 5 seconds with the camera positioned to take an image of all towers at once. Photos were merged into a movie using Picasa 3 (Google, Inc, Mountain View, CA) and climbing was quantified with Noldus Ethovision XTsoftware (Noldus Information Technology, Wageningen, Netherlands). Time spent climbing was defined as time visible in the hardware cloth tower. The center point detection setting was used to track the white mouse against the background of the dark room. The mice were detected as a moving white object that was 150–4000 pixels, allowing for changes in size with movement. The videos were sampled at 0.02 frames / s.
7
Elevated Plus Maze for Anxiety Assessment
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The Elevated Plus Maze was used to assess general anxiety behavior (Balsevich et al., 2014 (link)). A maze was placed 55 cm above floor with four black Plexiglas arms, two open arms (67 cm × 7 cm) and two enclosed arms (67 cm × 7 cm × 17 cm) which formed a cross shape opposing each open area. Adult mice were placed in the center of the maze and allowed to explore for 5 min. A camera mounted directly above the maze recorded the behavior of the animal and was analyzed by the image tracking system (Noldus Ethovision XT software; Noldus Information Technology, United States). Measurements included time spent in open and enclosed arms and number of entries in open areas.
8
Voluntary Wheel Running and Open-Field Behavior in Mice and Rats
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Mice were single housed with wireless low-pro le running wheels (Med Associates, Fairfax, VT) to measure voluntary wheel running activity, which was quanti ed as total number of rotations per night (day running is not reported as mice display minimal activity during the day). Running wheels were provided to mice for 10-12 days prior to the initial LPS or PBS treatment to allow the mice to develop stable baseline running behavior. Locomotor activity in a new environment was measured for 5 min after mice were individually placed in an empty rectangular arena (18.4 x 29.2 cm). Activity was recorded by video camera and distance traveled was quanti ed using Noldus Ethovision XT Software (Noldus Information Technology, Leesberg, VA).
Open-eld behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-eld box of 65 x 65 x 65 cm and lmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
Open-eld behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-eld box of 65 x 65 x 65 cm and lmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
9
Voluntary Wheel Running and Open-Field Behavior in Mice and Rats
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Mice were single housed with wireless low-pro le running wheels (Med Associates, Fairfax, VT) to measure voluntary wheel running activity, which was quanti ed as total number of rotations per night (day running is not reported as mice display minimal activity during the day). Running wheels were provided to mice for 10-12 days prior to the initial LPS or PBS treatment to allow the mice to develop stable baseline running behavior. Locomotor activity in a new environment was measured for 5 min after mice were individually placed in an empty rectangular arena (18.4 x 29.2 cm). Activity was recorded by video camera and distance traveled was quanti ed using Noldus Ethovision XT Software (Noldus Information Technology, Leesberg, VA).
Open-eld behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-eld box of 65 x 65 x 65 cm and lmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
Open-eld behavioral testing of rats was performed 2 and 24 h after LPS administration. Each rat was placed into an open-eld box of 65 x 65 x 65 cm and lmed for 7 min. The video was analyzed using Ethovision. The arena was divided into two zones: a central zone and an edge zone. The frequency of center entries was assessed as a measure of anxiety and the distance covered per minute and total distance covered were assessed as measures of locomotor activity. The arena was thoroughly cleaned 70% ethanol between trials and animals.
10
Forced Swim Test for Behavioral Despair
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This procedure provides an index of “behavioral
despair” based on the profile of mobility during a forced swim
session.(Porsolt et al., 2001 (link)) In
this procedure, mice were brought to the testing room to acclimate 1h prior
to testing. A constant white noise is set at 65dB using a generator (San
Diego Instruments) to mask extraneous sounds that might influence swim
behavior. For testing, each mouse is gently placed into the testing cylinder
(40 cm height, 20 cm diam, Stoelting) with water level at 25 cm and
temperature adjusted to 23–24 °C for a single 6-min session.
Two cameras (one overhead and one side facing at water level) were
positioned to video record each swimming session. Noldus Ethovision XT
software (v13.5) was used to automatically track and score for activity and
immobility.(Bambico et al.,
2007 )
despair” based on the profile of mobility during a forced swim
session.(Porsolt et al., 2001 (link)) In
this procedure, mice were brought to the testing room to acclimate 1h prior
to testing. A constant white noise is set at 65dB using a generator (San
Diego Instruments) to mask extraneous sounds that might influence swim
behavior. For testing, each mouse is gently placed into the testing cylinder
(40 cm height, 20 cm diam, Stoelting) with water level at 25 cm and
temperature adjusted to 23–24 °C for a single 6-min session.
Two cameras (one overhead and one side facing at water level) were
positioned to video record each swimming session. Noldus Ethovision XT
software (v13.5) was used to automatically track and score for activity and
immobility.(
2007
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