Primary human glomerular microvascular endothelial cells (hgMVECs) were purchased at Cell-System (Kirkland, WA, USA; ACBRI-128). Cells were cultured at 37 °C with 5% CO2 in full endothelial growth medium-2 (EGM-2) supplemented with 100 IU/mL penicillin and 100 μg/mL streptomycin. For the experiments, cells were left attached for 24 h and then treated for 24 h or 48 h with sodium butyrate (Sigma, St. Louis, MI, USA, 303410) or sodium acetate (Sigma, St. Louis, MI, USA; S2889-250G), or for 24 h with sodium butyrate/sodium acetate and 100 ng/mL of lipopolysaccharides from E. Coli O111:B4 (Sigma, St. Louis, MI, USA; L4391).
Sodium acetate
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Spain, India, Italy, France, China, Poland, Canada, Australia, Ireland, Hungary, Mexico, Chile, Switzerland, Brazil, Macao, Netherlands, Belgium, New Zealand, Portugal, Czechia, Sweden, Sao Tome and Principe
Sodium acetate is a chemical compound with the formula CH3COONa. It is a common salt that is widely used in various laboratory and industrial applications. Sodium acetate functions as a buffer solution, helping to maintain a specific pH level in chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Acetic acid, by Merck Group (226 mentions)
Hydrochloric acid (hcl), by Merck Group (177 mentions)
Sodium hydroxide (naoh), by Merck Group (162 mentions)
Gallic acid, by Merck Group (145 mentions)
Methanol, by Merck Group (140 mentions)
Sodium carbonate, by Merck Group (134 mentions)
Sodium chloride (nacl), by Merck Group (107 mentions)
Ethanol, by Merck Group (101 mentions)
Acetonitrile, by Merck Group (86 mentions)
Quercetin, by Merck Group (84 mentions)
Glacial acetic acid, by Merck Group (82 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (81 mentions)
Potassium chloride (kcl), by Merck Group (80 mentions)
Formic acid, by Merck Group (80 mentions)
Folin ciocalteu reagent, by Merck Group (78 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (76 mentions)
Ascorbic acid, by Merck Group (58 mentions)
Potassium persulfate, by Merck Group (53 mentions)
Catechin, by Merck Group (49 mentions)
Acetone, by Merck Group (47 mentions)
1 179 protocols using sodium acetate
1
Culturing and Treating hgMVECs
2
Synthesis and Characterization of NAX 810-2
3
Sodium Acetate in PCOS Intervention
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The control group received vehicle (distilled water, p.o.), SATE group received sodium acetate (200 mg/kg, p.o.,) obtained from Sigma-Aldrich, St Louis, MI, PCOS group received distilled water and PCOS + SATE group received sodium acetate and the treatment lasted for six weeks [10 (link)].
4
Manganese Electroplating Protocol
5
Antioxidant and Phytochemical Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sulfuric acid, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), sodium thiosulphate, Folin–Ciocalteu reagent (2N), aluminum chloride, sodium hydroxide, sodium acetate, gallic acid potassium chloride, aluminum chloride, sodium hydroxide, ferric chloride heptahydrate, potassium carbonate, sodium acetate, methanol, pectin, whey protein isolates, and sodium caseinate were obtained from Sigma Aldrich (St. Luis, MO, USA).
6
Synthesis of Triphenylamine Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Triphenylamine (98%), phosphorus(v ) oxychloride (99%), sodium borohydride (96%), sodium acetate (99%), triethylamine (anhydrous, 99.5%), acryloyl chloride, sodium acetate, 2,2′-azobis(2-methylpropionitrile) (AIBN, 98%), 4-methoxyTriphenylamine (97%), iron(iii ) chloride (97%), N,N-dimethylformamide (DMF, 99.8%), methyl alcohol (99.8%), 1-methyl-2-pyrrolidinone (NMP, 99%) and anhydrous chloroform (99%) were all purchased from Sigma Aldrich and used as received. Tetrahydrofuran (Boom) and toluene (Macron) were dried using the MBraun SPS800 system. Carboxylic-acid functionalized multiwall carbon nanotube (MWCNT-COOH, 9.5 nm × 1.5 micron) was also purchased from Aldrich and used with no further treatment.
7
Phytochemical Extraction and Antioxidant Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acetic acid, acetonitrile, methanol, gallic acid, rosmarinic acid, rutin, hydrochloric acid, sodium acetate, quercetin, Folin–Ciocâlteu reagent, protocatequic acid, gentisic acid, chlorogenic acid, catechin, caffeic acid, elagic acid, sinapic acid, ferulic acid, myricetin, tilirosid, quercetin, trans-cinnamic acid, luteolin were purchased from Sigma-Aldrich (Steinheim, Germany). Aluminum chloride, sodium acetate, sodium carbonate, ethanol was purchased from Merck, Darmstadt, Germany and DPPH (2,2-diphenyl-1-picrylhydrazyl) and BHT (butylated hydroxytoluene) were obtained from Alfa-Aesar (Germany). All microorganism strains were distributed by Oxoid®. All spectrophotometric data were acquired using a Jasco V-530 UV-Vis spectrophotometer (Jasco International Co., Ltd., Tokyo, Japan).
8
Letrozole-Induced PCOS Model with Sodium Acetate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Distilled water was given as vehicle to the control group, NaAc group received 200 mg/kg of sodium acetate (Sigma-Aldrich, St Louis, MI) [24 (link),29 (link)], PCOS group received 1 mg/kg of letrozole (Sigma-Aldrich, St Louis, MI) and PCOS+NaAc group received letrozole and sodium acetate. The administrations were done uninterruptedly by oral gavage for 21 days [8 (link),26 (link),27 (link)]. Initial and final body weights were monitored, and the body weight gain was estimated.
9
Antioxidant Activity Assessment of Melatonin Formulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melatonin was purchased from Methapharmaceutical IND S.L. (Spain). Mannitol, polyvinylpyrrolidone (crospovidone), lactose, magnesium stearate, anhydrous colloidal silica, tartaric acid and sodium bicarbonate were all supplied by Fagron Ibérica S.A.U. (Spain).
The antioxidants, vitamin C (sodium ascorbyl phosphate) and resveratrol were supplied by Fagron Ibérica S.A.U. (Spain). NADPH, adenosine triphosphate (ATP) and glutathione (GSH) were purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the ferric ion reducing antioxidant power (FRAP) technique, the following were used: sodium acetate, acetic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydrochloric acid, ferric chloride, FeSO4 × 7 H2O and bidistilled water, purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the TAC technique, the following were used: sodium acetate, bidistilled water, glacial acetic acid, hydrogen peroxide, 2,2’-azino-bis-2-ethybenzothiazoline-6-sulphonic acid (ABTS), the water-soluble analogue of vitamin E (Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)), ethanol, methanol and monosodium phosphate, purchased from Sigma Aldrich (Spain).
The antioxidants, vitamin C (sodium ascorbyl phosphate) and resveratrol were supplied by Fagron Ibérica S.A.U. (Spain). NADPH, adenosine triphosphate (ATP) and glutathione (GSH) were purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the ferric ion reducing antioxidant power (FRAP) technique, the following were used: sodium acetate, acetic acid, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), hydrochloric acid, ferric chloride, FeSO4 × 7 H2O and bidistilled water, purchased from Sigma Aldrich (Spain).
For the determination of total antioxidant activity using the TAC technique, the following were used: sodium acetate, bidistilled water, glacial acetic acid, hydrogen peroxide, 2,2’-azino-bis-2-ethybenzothiazoline-6-sulphonic acid (ABTS), the water-soluble analogue of vitamin E (Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid)), ethanol, methanol and monosodium phosphate, purchased from Sigma Aldrich (Spain).
10
Optimizing Nitrogen Source Concentration for Microbial Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of different concentrations of N source was determined at 11.89, 27.84, 36.20 and 44.55 (g/L) of yeast extract (Ohly KAT, Germany). The concentration (g/L) of glucose was fixed at 20.0, sodium acetate at 5.0, diammonium hydrogen citrate at 2.0, dipotassium hydrogen phosphate at 2.0, Tween 80 at 1.0, magnesium sulphate heptahydrate at 0.2 and manganese sulphate tetrahydrate at 0.04, which followed the composition of MRS broth (Table 8 ; Merck, Darmstadt, Germany). glucose, sodium acetate, diammonium hydrogen citrate, dipotassium hydrogen phosphate, Tween 80, magnesium sulphate heptahydrate and manganese sulphate tetrahydrate were purchased from Merck Company (Darmstadt, Germany). The experiment was performed in triplicate with 2 h sampling intervals.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!