Anti gfp

The following antibodies were used: anti-β-actin, anti-G6PD (Sigma-Aldrich); anti-HBcAg (Abcam, Cambridge, MA, USA), anti-Keap1 (Cell Signaling, Danvers, MA, USA); anti-Myc, anti-Nrf2 (sc-365949), anti-Lamin B, anti-p62, and anti-Flag (Santa Cruz, Santa Cruz, CA, USA); anti-GFP (BD Biosciences, Franklin Lakes, NJ, USA); goat anti-rabbit IRDye800CW and goat anti-mouse IRDye680 (LI-COR Biosciences, Lincoln, NE, USA). Plasmids with the 1.3mer HBV genomic DNA (pUC19 HBV), the HBx-negative 1.3mer HBV genomic DNA (pUC19 HBVX⁻) and HBx-GFP were described previously.^{19 (link), 36 (link)} Dsred-Keap1 was provided by Yue Xiong (University of North Carolina at Chapel Hill, Chapel Hill, NC, USA). For Flag-p62 construction, the p62 coding region was amplified from Cherry-GFP-p62, a gift from Terje Johansen (University of Tromsø, Tromsø, Norway), and was inserted into pCMV-Tag 2A vector using the EcoRI and XhoI restriction sites. HBx-Myc was made by cloning the cDNA of HBx into pcDNA-3.1 vector using EcoRI and XhoI restriction sites. The point mutation for Flag-p62 T350A and Dsred-Keap1 N382A was created by PCR-based site-directed mutagenesis using primer: 5′-AAGAAGTGGACCCGTCTGCAGGTGAACTCCAGTCC-3′ (p62-sense), 5′-GGACTGGAGTTCACCTGCAGACGGGTCCACTTCTT-3′ (p62-antisense), 5′-CGTGGGCGGCAGGGCCAACTCGCCCGAC-3′ (Keap1-sense) and 5′-GTCGGGCGAGTTGGCCCTGCCGCCCACG-3′ (Keap1-antisense).

Most general reagents used in this study were sourced from Sigma-Aldrich. The siRNA oligonucleotides were purchased from Dharmacon. Primary antibodies used in this study were as follows: anti-TBC1D5 [Santa Cruz, sc-376296, dilution 1:400 or 1:1000 for immunofluorescence (IF) microscopy or western blotting (WB), respectively], anti-VPS26 (Abcam, ab23892, 1:800 IF or 1:1000 WB), anti-VPS35 [Santa Cruz, sc-374372, 1:800 IF or 1:1000 WB, or from the Seaman lab (see Seaman, 2007 (link)), 1:300 for IF], anti-CIMPR (Abcam, ab2733, 1:400 IF or 1:1000 WB), anti-Lamp1 (Santa Cruz, sc-18821, 1:500 IF or 1:1000 WB), anti-Glut1 (Abcam, ab15309, 1:400 IF), anti-GM130 (BD Transduction labs 610822, 1:500 IF), anti-Fam21 (Millipore, ABT79, 1:400 IF or 1:1000 WB), anti-Aβ (Covance, SIG-39320, 1:1000 WB), anti-sAPPβ (IBL America, 10321, 1:800 WB), anti-Rab7a:GTP (NewEast Biosciences, 26923, 1:300 IF), anti-TGN46 (Seaman lab, see Seaman, 2007 (link), dilution 1:600 IF), anti-GFP (Seaman lab, see Seaman et al., 2009 (link), 1:1000 for immunoprecipitation), anti-Snx1 (BD Transduction labs, dilution 1:400 IF or 1:1000 WB) and anti-Tubulin (Sigma-Aldrich, dilution 1:1000 WB). Secondary fluorescently labelled antibodies were purchased from Invitrogen.