Dulbecco s modified eagle medium dmem cell culture

Ferric chloride, sodium acrylate, sodium acetate, ethylene glycol (EG), diethylene glycol (DEG), tetraethyl orthosilicate (TEOS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), dimethyl sulfoxide (DMSO), and poly-acrylic acid (PAA) were obtained from the Merck Company (Germany). Dulbecco’s Modified Eagle Medium (DMEM) cell culture, MTT powder, and fetal bovine serum (FBS) were purchased from Gibco (Scotland). Lf and Doxo were purchased from the Sigma Aldrich Company (USA). Annexin V-FITC kit was obtained from Iq products, Poland. The BALB/c mice and 4T1 cells were purchased from Pasteur Institute (Tehran, Iran).

CNTs with <99% purity (SWCNT: 1–2 nm length, MWCNT: 20–30 nm length) were purchased from Notrino Co (St. Sadeghiye, Tehran, Iran). The MC4L2 cell line was purchased from Iranian Biological Resource Center (Tehran, Iran). Dulbecco’s Modified Eagle Medium (DMEM) cell culture and FBS were purchased from Scotland (Gibco, Scotland). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) powder and Doxorubicin were purchased from Sigma Co. Annexin kit was purchased from Iq products, Poland. Ketamine and Xylazine were purchased from (Sigma Aldrich Co, USA). The BALB/C mouse was purchased from Iran Pasteur Institute (Tehran, Iran). TRIzol solution and 2× Real Rime SYBER GREEN Master Mix were purchased from Invitrogen, USA. The first standard cDNA synthesis kit and nuclease-free water were purchased from Representing of Fementas Co. (USA).