Magnesium chloride

Potassium PFOS (99% pure) was purchased from AccuStandard. HPLC-grade methanol and acetonitrile were purchased from Fisher Scientific. Sodium chloride, magnesium chloride, iron chloride, sodium hydroxide, and hydrochloric acid were purchased from Sinopharm Chemical Reagent Company. Biphenyl (99% pure) was provided by Aladdin. Other materials were purchased from commercial suppliers and used without further purification unless otherwise noted. The ultrapure water used was produced using a Milli-Q integral water purification system.
The morphology of the PAF-45 was examined using a Hitachi S-3000N field emission scanning electron microscope and a JEOL JEM-2100F transmission electron microscope. The crystallinity and regularity of the PAF-45 were determined by X-ray diffractometry using an AXS instrument. The surface area of the PAF-45 was determined using the Brunauer, Emmett, and Teller method. The functional groups were identified using Fourier transform infrared spectroscopy. The zeta potential was determined at different pH values. The pH was measured using a pH meter. The contact angle of the PAF-45 was determined using a DataPhysics DCAT21 dynamic contact angle measuring instrument.

Myriophyllum verticillatum (MV) was collected from Yanghu Wetland Park, Changsha, China, and after collection, it was washed several times with deionized water, dried naturally in the sun, and then dried in an oven at 60 °C for 24 h. The dried Myriophyllum verticillatum was then made into powder using a pulverizer and passed through a 70-mesh sieve. Triphenyl phosphate (TPhP) and sodium periodate (PI) were purchased from Shanghai McLean Biochemical Co. Manganese acetate, potassium permanganate, magnesium chloride, potassium dihydrogen phosphate, potassium persulfate, ascorbic acid, ammonium molybdate, and potassium antimony tartrate were purchased from Shanghai Sinopharm Chemical Reagent Co. Methanol, hydrochloric acid and sodium hydroxide were purchased from Tianjin Hengxing Chemical Reagent Manufacturing Co. HPLC-grade acetonitrile was purchased from Shanghai Aladdin Biochemical Technology Co. All chemicals were analytically pure, and ultrapure water (≥18.25 MΩ·cm) was prepared using an ultrapure water machine at the College of Environmental Science and Engineering, Central South University of Forestry Science and Technology.

Koumine was isolated from G. elegans by one of the authors (Yanping Su) using the method reported earlier (Su et al. 2011 (link)) and the purity reached 99.0%. Hydroxypropyl-β-cyclodextrin (HP-β-CD) was a gift sample from Shandong Qianhui Biotechnology Co. Ltd., China. Dulbecco’s modified Eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from HyClone, USA. HBSS buffer was purchased from Shanghai Baisai Media Technologies Co. Ltd., China. Sodium chloride, potassium chloride, calcium chloride, sodium bicarbonate, potassium dihydrogen phosphate, glucose, and magnesium chloride were all obtained from Sinopharm Reagents Co. Ltd., China. All organic solvents were of analytical grade unless otherwise specified.

Scoured and bleached cotton woven fabric (133 warp yarns and 100 wefts per 10 cm, weighs 133 g/m²) was purchased from Shandong Lutai Group Co., Ltd. Zibo, China. Sucrose (Su), hydroxyl amine hydrochloride, magnesium chloride (MgCl₂), sodium hypophosphite (SHP), NaIO₄, and GA were purchased from Sinopharm Chemical Reagent Co., Ltd. Shanghai, China. TEMPO, Laccase (0.5U/mg), DMDHEU and BTCA were purchased from J&K Scientific Ltd. Beijing, China. All chemical reagents were of reagent grade.

PVA (M_w = 1700, 99% alcoholysis) and EC (47% ethyl) were purchased from Shanghai Yuanye Biotechnology Co. Potassium nitrate (KNO₃, 99.5%), sodium chloride (NaCl, 99.5%), sodium bromide (NaBr, 99.5%), potassium carbonate (K₂CO₃, 99.5%), magnesium chloride (MgCl₂, 99.5%), lithium chloride (LiCl, 98%), and Tween-80 (98%) were purchased from Sinopharm Chemical Reagent Co. Anhydrous ethanol (99.7%) and glacial acetic acid (99.5%) were purchased from Chengdu Kolon Reagent Company (China). Eo (98%) was purchased from Shanghai Aladdin Biochemical Technology Co. All reagents were used without further purification.

Pumpkin seeds (Cucurbita pepo L.) were provided by Sanxin Company (Ürümqi, Xinjiang, China). Reagents used in this study were of analytical grade unless otherwise specified. N-hexane was purchased from InnoChem Science and Technology Co., Ltd. (Beijing, China). Disodium hydrogen phosphate dodecahydrate, sodium phosphate dibasic dihydrate, disodium tetraborate decahydrate, O-phthalic aldehyde (OPA), dithiothreitol (DTT), serine, folin-phenol, sodium carbonate, trichloroacetic acid (TCA), absolute ethyl alcohol, sodium chloride, sodium hydroxide, calcium chloride, potassium chloride, sodium bicarbonate, and magnesium chloride, were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Coomassie Blue Fast Staining Solution G250, Bovine Serum Albumin (BSA), and Phosphate-Buffered Saline (PBS) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).