To calculate short-term in vivo biological safety, mice were administered with free curcumin, ExoCTP and Cur@ExoCTP on Days 0, 1, 2 and 7 via tail vein injection, and serum was collected on Days 3 and 8. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (CRE) were detected with respective detection kits (BC1555, BC1565, BC1535 for Solarbio or F10315 for Westang Biotech) as described previously [28 (link), 33 (link)]. To investigate long-term in vivo biosafety, major organs, including the liver, kidney, lung, spleen and brain were H&E-stained for morphology assay on Day 28 post-injection [34 (link), 35 ].
Bc1535
Manufactured by Solarbio
Sourced in China
The BC1535 is a benchtop centrifuge designed for general laboratory use. It is capable of processing a variety of sample types and volumes. The centrifuge features a simple and intuitive control interface for ease of use.
Automatically generated - may contain errors
4 protocols using bc1535
1
In Vivo Biological Safety Evaluation
2
Biochemical Markers in Serum Analysis
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Serum uric acid (BC1365, Solarbio, China), serum creatinine (BC4915, Solarbio, China), and blood urea nitrogen (BC1535, Solarbio, China) were prepared according to the reagent instructions (Multiskan GO, Thermo, USA) to determine the absorbance of each sample.
3
Metabolic Cage Assessment of Renal Function
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A metabolic cage was used to collect urine. Urinary volume, urinary glucose excretion (BC2505; Solarbio), urinary albumin (MM-44287M1; MEIMIAN), and urinary creatinine (C011-2-1; Nanjing JianCheng Bioengineering Institute) were measured every 2 weeks. At the end of the 12-week intervention, blood urea nitrogen (BUN) (BC1535; Solarbio) and creatinine (C011-2-1; Nanjing JianCheng Bioengineering Institute) were determined by biochemical methods, and the kidneys were weighed.
4
Renal Biomarkers in Rat Serum
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Peripheral blood samples were collected and centrifuged (3000 rpm for 10 min) to separate serum. The levels of blood urea nitrogen (BUN; BC1535, Solarbio, Beijing, China), creatinine (Cr; MAK080, MO, USA), creatine kinase (CK; E-El-R1322c, Houston, USA), and kidney injury molecule 1 (Kim-1; E-EL-R3019, Houston, USA) in rat serum in the control, CP, CP + Dex 10 μg/kg, and CP + Dex 25 μg/kg groups were detected.
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