All general reagents used for cell culture were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA), including the cell culture media and culture dishes. The protease inhibitors were purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals were purchased from Sinopharm Chemicals (Beijing, P. R. China). QDB plates were manufactured by Quanticision Diagnostics, Inc. (RTP, USA). Mouse anti-Ki67 antibody (clone MIB1) was purchased from ZSGB-BIO (Beijing, China). HRP-labeled Donkey Anti-Mouse IgG secondary antibody was purchased from Jackson Immunoresearch lab (Pike West Grove, PA, USA).
PCR reagents, restriction enzymes, and T4 DNA ligase were purchased from Takara Bio Inc. (Dalian, China). Competent cells Escherichia coli DH5a and BL21(DE3) were from TransGen Biotech (Beijing, China). IPTG (Isopropyl β-D-1-thiogalactopyranoside) was purchased from Solarbio (Beijing, China). Nickel-His GraviTrap affinity column was purchased from GE Healthcare.
PCR reagents, restriction enzymes, and T4 DNA ligase were purchased from Takara Bio Inc. (Dalian, China). Competent cells Escherichia coli DH5a and BL21(DE3) were from TransGen Biotech (Beijing, China). IPTG (Isopropyl β-D-1-thiogalactopyranoside) was purchased from Solarbio (Beijing, China). Nickel-His GraviTrap affinity column was purchased from GE Healthcare.