Serum concentrations of hsCRP and high-sensitive interleukin 6 (hsIL-6) were determined by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's specifications (hsCRP: CYT298, Millipore, Billerica, Massachusetts; hsIL-6: R&D Systems, Minneapolis, Minnesota). Assays sensitivity and intra-/inter-assay variability were, respectively, 0.20 ng/mL, ± 4.6% and ± 6.0% for hsCRP and 0.031 pg/mL, ± 4.1% and ± 3.9% for hsIL-6.
Cyt298
Manufactured by Merck Group
Sourced in United States
CYT298 is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and analysis. The core function of CYT298 is to provide accurate and reliable measurements for various applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using cyt298
1
Quantifying Inflammatory Biomarkers
2
Measuring Systemic Inflammation via hsCRP
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Fasting blood samples were collected for the measurement of serum concentrations of high-sensitive C-reactive protein (hsCRP) as a marker of systemic inflammation. After 30–45 min at room temperature, samples were centrifuged (3200 rpm, 10 min at 4 °C) and stored at −80 °C until further analysis. Serum concentrations of hsCRP were determined by ELISA according to the manufacturer's specifications (CYT298, Millipore, Billerica, Massachusetts). Assays sensitivity and intra- and inter-assay variability were respectively 0.20 ng/mL, ± 4.6%, and ±6.0%.
3
Bariatric Surgery Candidate Evaluation
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Clinical and biological assessments occurred at the clinic during a medical visit with surgeons to discuss the possibility and modalities of future bariatric surgery.
Socio-demographic, clinical characteristics and blood samples were collected during this visit. BMI was calculated as self-reported weight (kg)/height (m) 2 .
Depressive symptoms were assessed using the Montgomery-Asberg Depression Rating Scale (MADRS), a 10-item clinician-administered scale assessing depressive symptoms, including sadness, inner tension, concentration difficulties, lassitude, inability to feel, pessimistic thoughts, suicidal thoughts, reduced sleep and appetite (15).
Fasting blood samples were collected in plain or EDTA-containing tubes for serum and plasma, respectively. After 30-45min at room temperature, samples were centrifuged Serum concentrations of high-sensitive C-reactive protein (hsCRP) were determined by enzyme-linked immunosorbent assay (CYT298, manufacture: Millipore, Billerica, Massachusetts, USA). Assay sensitivity and intra-/inter-assay variability were, respectively, 0.20 ng/mL, ± 4.6%, and ± 6.0%.
Socio-demographic, clinical characteristics and blood samples were collected during this visit. BMI was calculated as self-reported weight (kg)/height (m) 2 .
Depressive symptoms were assessed using the Montgomery-Asberg Depression Rating Scale (MADRS), a 10-item clinician-administered scale assessing depressive symptoms, including sadness, inner tension, concentration difficulties, lassitude, inability to feel, pessimistic thoughts, suicidal thoughts, reduced sleep and appetite (15).
Fasting blood samples were collected in plain or EDTA-containing tubes for serum and plasma, respectively. After 30-45min at room temperature, samples were centrifuged Serum concentrations of high-sensitive C-reactive protein (hsCRP) were determined by enzyme-linked immunosorbent assay (CYT298, manufacture: Millipore, Billerica, Massachusetts, USA). Assay sensitivity and intra-/inter-assay variability were, respectively, 0.20 ng/mL, ± 4.6%, and ± 6.0%.
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