Originpro 2017

Data are presented as means ± SEM. All statistical analyses were performed with OriginPro 2017 and GraphPad Prism 8 (GraphPad Software). Before ANOVA, a one-sample Kolmogorov-Smirnov test was done with all data groups to verify whether they were distributed normally. Statistical significance between two eyes of the same animal was determined by paired t test, whereas unpaired t test or one-way ANOVA was applied when comparing eyes from different mice. Difference in growth curves and that in I-R curves were compared with two-way ANOVA followed with Sidak post hoc multiple comparisons tests. Significant difference was defined as a P value smaller than 0.05.

To assess the percentage of NK cells in the collected PBMCs, the samples were analyzed by flow cytometry using anti-human monoclonal antibodies: CD3 (clone BW264/56, PerCP; Miltenyi Biotec #130-096-910), CD4 (Clone VIT4; label APC-Vio770; Miltenyi Biotec # 130–098-153), CD8 (clone RPA-T8, PE; Beckton Dickinson) and CD56 (clone AF12-7H3, PE-Vio770; Miltenyi Biotec #130-096-831). Samples were acquired on Becton Dickinson, Allschwil, Switzerland FACSCanto II instrument. Flowjo V 10.0.8r1, Becton Dickinson and Company, Franklin Lakes, NJ, USA, Origin Pro 2017, Northampton, MA, USA and GraphPad Prism 5.0 Software, San Diego, CA, USA were used for data analysis and statistics.