Pd minitrap g 25
The PD MiniTrap G-25 is a pre-packed size exclusion chromatography column designed for desalting and buffer exchange of small sample volumes. The column comes pre-packed with Sephadex G-25 resin and is available in two sizes, 1 mL and 5 mL, to accommodate different sample volumes.
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6 protocols using pd minitrap g 25
VNAR Antibody Expression and Purification
Measuring IscS-IscU Binding Affinity
Antibody-Drug Conjugate Formation via Cysteine Engineering
Kinesin Protein Labeling and Storage
Preparation and Characterization of Fluorescent Liposomes
Liposome Scrambling and Leakage Assays
For the NBD-glucose leakage control assay43 (link), the lipid film was equilibrated with NBD-glucose-containing buffer (12.6 µM). Excess NBD-glucose was removed by passing the liposomes through a desalting column (PD MiniTrap G25, Cytiva) pre-washed with NBD-glucose-free buffer. Next, the experimental procedure was performed similarly to the scrambling assay, with first dithionite addition (to 1 mM), following second dithionite addition and (1 mM) and Triton X-100 (0.5% w/v) to quench the NBD-glucose signal completely.
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