Intelligent uv vis detector

The content of naringin was determined using a previously published validated RP-HPLC method [11 (link)]. All test samples were diluted suitably with mobile phase, and the chromatographic separation was performed using an isocratic elution. The mobile phase consisted of a mixture of potassium phosphate buffer (10 mM, pH adjusted to 3.6 using dilute orthophosphoric acid) and acetonitrile (25:75) and delivered at a flow rate of 1 mL/min. The HPLC system consisted of a pump (Jasco PU-2080 Plus, Intelligent HPLC pump, Jasco, Tokyo, Japan) connected to Detector (Jasco 2075, Intelligent UV–vis detector, Jasco, Tokyo, Japan). The separation was carried out at 20 °C, on a reversed-phase C18 HPLC column (Qualisil^® BDS, 250 mm × 4.6 mm, 5 μm particle size, Qualisil, Agilent Technologies, Mumbai, Maharashtra, India). An injection volume of 20 μL was used. Detections were carried out at 284 nm.

Naringin content was estimated using the validated RP-HPLC method (Supplementary Data File, Section S5 HPLC method validation and Table S3). All test samples were diluted suitably with mobile phase and the chromatographic separation was performed using an isocratic elution. The mobile phase consisted of a mixture of potassium phosphate buffer (10 mM, pH adjusted to 3.6 using dilute orthophosphoric acid) and acetonitrile (25:75) and delivered at a flow rate of 1 mL/min. The HPLC system consisted of a pump (Jasco PU-2080 Plus, Intelligent HPLC pump, Tokyo, Japan) connected to Detector (Jasco 2075, Intelligent UV–vis detector, Tokyo, Japan). The separation was carried out at 20 °C, on a reversed-phase C18 column (Qualisil^® BDS, 250 × 4.6 mm, 5 μm particle size). An injection volume of 20 μL was used. Detections were carried out at 284 nm. The method was validated for accuracy, precision, specificity and solution stability.

Encapsulation efficiency was analyzed by the validated RP-HPLC method reported earlier with slight modification [22 (link),23 (link)]. The RP-HPLC analysis was carried out at 22 °C (Jasco PU-2080 Plus, Intelligent HPLC pump, Japan, Detector of Jasco 2075, Intelligent UV–vis detector, Jasco, Tokyo, Japan, and RP-C18 column of Agilent, 250 × 4.6 mm, 5 μm particle size, Technologies, Mumbai, India). 20 μL of each sample was injected after suitable dilution with mobile phase and detected at 210 nm. The mobile phase consisted of phosphate buffer pH 5.5 and acetonitrile (25:75), and an isocratic flow of 1 mL/min was used [23 (link)].