The recombinant proteins used in this study were, for mouse: Integrin α4β1 (R&D cat 7810-A6-050); Integrin α5β1 (R&D cat 7728-A5-050); Integrin αVβ1 (R&D cat 7705-AV-050); Integrin α6β1 (R&D cat 7810-A6-050); TGFβ1 (R&D Systems cat 7666-MB-005); Laminin (Invitrogen cat 23017-015). For human: Integrin α4β1 (R&D cat 5668-A4-050); Laminin (BioLamina cat LN-211); Collagen (Sigma-Aldrich cat C2249).
Integrin α6β1
Manufactured by R&D Systems
Sourced in United States
Integrin α6β1 is a cell surface receptor that mediates attachment to the extracellular matrix. It functions as a laminin receptor and is involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Integrin α4β1, by R&D Systems (3 mentions)
Integrin α5β1, by R&D Systems (3 mentions)
Laminin, by Thermo Fisher Scientific (2 mentions)
Integrin αvβ1, by R&D Systems (2 mentions)
Laminin, by BioLamina (2 mentions)
Tgf β1, by R&D Systems (2 mentions)
Cellquest pro software, by BD (1 mentions)
Integrin α3β1, by R&D Systems (1 mentions)
E cadherin, by BioLegend (1 mentions)
3 protocols using integrin α6β1
1
Integrin and Extracellular Matrix Proteins
2
Integrin and Extracellular Matrix Proteins
3
Characterization of Cellular Surface Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HTR8/SVneo, JEG3, JAR and BeWo cells were treated with trypsin and transferred into plastic tubes after cultured at a density of 2×105 cells/well in 6-well microplates for 48 h. Cells were fixed in 4% paraformaldehyde for 20 min at room temperature, washed in PBS and permeabilized for 20 min in 0.1% Triton X-100-PBS. They were then washed and suspended in PBS, incubated with ST2-allophycocyanin-labeled antibodies (R&D Systems, Inc., Minneapolis, MN, USA) for 30 min at room temperature. Next, cells were washed and suspended in PBS, and immediately analyzed by four-color FCM using CellQuest Pro software, version 5.1 (FACSCalibur; BD Biosciences, Franklin Lakes, NJ, USA) with an isotypic control (BioLegend, San Diego, CA, USA).
JEG3 cells were cultured at a density of 2×105 cells/well in 6-well microplates, and treated with recombinant human (rh)IL-33 (at 0 and 1 ng/ml) for 48 h, then the expression of integrin α3β1, integrin α4β1, integrin α5β1, integrin α6β1 and integrin ανβ3 (R&D Systems, Inc.), CD44, CD62L and E-cadherin (BioLegend, Inc., San Diego, CA, USA) on JEG3 cells was evaluated by FCM.
JEG3 cells were cultured at a density of 2×105 cells/well in 6-well microplates, and treated with recombinant human (rh)IL-33 (at 0 and 1 ng/ml) for 48 h, then the expression of integrin α3β1, integrin α4β1, integrin α5β1, integrin α6β1 and integrin ανβ3 (R&D Systems, Inc.), CD44, CD62L and E-cadherin (BioLegend, Inc., San Diego, CA, USA) on JEG3 cells was evaluated by FCM.
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